ABN242

Anti-Pannexin-1/PANX1 Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Pannexin-1, Innexin
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human, mouse, rat
• reaktywność gatunkowa (przewidywana na podstawie homologii): gorilla (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• metody: immunohistochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NP_056183
• numer dostępu UniProt: Q96RD7
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... PANX1(24145)

Opis

Opis ogólny

Pannexin-1 (UniProt Q96RD7; also known as Innexin) is encoded by the PANX1 (also known as MRS1, PX1) gene (ORF UNQ2529/PRO6028; Gene ID 24145) in human. Pannexin 1 is a high-conductance, voltage-gated channel protein widely expressed in many organs and tissues, including the sensory systems (eye, inner ear, taste buds, and the olfactory epithelium) and both neuronal and non-neuronal cell types. Three pannexin family genes (PANX1-3) have been identified in human and mouse genomes. Pannexin proteins share structural and functional similarities and play important roles in many physiological and pathological processes, such as ATP release, Ca2+ homeostasis, synaptic neurotransmitters release, apoptosis, and immunological response. Pannexin-1 deficiency causes caspase-3 activation and apoptosis induction of hair cells in cochlear spiral limbus (SLM), resulting in hearing loss among Panx1-/- mice. Panx1 is also expressed in the retina, lens and cornea of the eye, where it plays an important role in mediating the crosstalk between immune and neuronal cells. Pannexin-1 channel opening, frequently together with the activation of purinergic receptors by extracellular ATP, is reported to have a great implication in diverse pathological conditions, including ischemia, trauma, HIV infection, inflammation, migraine, and cancer cell growth.

Specyficzność

Expected to react with both spliced isoforms reported by UniProt (Q96RD7-1 & Q96RD7-2).

Immunogen

Epitope: C-terminal

GST-tagged recombinant human Pannexin-1/PANX1 C-terminal fragment.

Zastosowanie

Anti-Pannexin-1/PANX1 Antibody is an antibody against Pannexin-1/PANX1 for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Pannexin-1/PANX1 in human testis and mouse kidney tissue sections.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Jakość

Evaluated by Western Blotting in human cortex tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Pannexin-1/PANX1 in 10 µg of human cortex tissue lysate.

Opis wartości docelowych

~55 kDa observed. 55-58 kDa (membrane glycosylated) and 43 kDa (cytosolic non-glycosylated) expected. 48.1 kDa (isoform 1) & 47.6 kDa (isoform 2) calculated.

Powiązanie

Replaces: AB9886

Postać fizyczna

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable