Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Key Documents

ABF1063

Sigma-Aldrich

Anti-SARS-CoV-2 spike protein S2 domain

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

białko sprzężone

unconjugated

forma przeciwciała

purified antibody
serum

rodzaj przeciwciała

primary antibodies

klon

polyclonal

masa cząsteczkowa

calculated mol wt 139.13 kDa
observed mol wt ~67 kDa

oczyszczone przez

(Unpurified)

reaktywność gatunkowa

virus

opakowanie

antibody small pack of 100 μL

metody

ELISA: suitable
neutralization: suitable
western blot: suitable

sekwencja epitopowa

C-terminal half

numer dostępu Protein ID

numer dostępu UniProt

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

vaccinia virus ... s> S(43740568)

Opis ogólny

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. (Ref.: Ravichandran, S., et al. (2020). Sci. Transl. Med. 12(550); eabc3539).

Specyficzność

This rabbit polyclonal antibody detects the S2 domain of SARS-CoV-2 spike protein.

Immunogen

His-tagged recombinant fragment corresponding to the S2 domain from the extracellular region of SARS-CoV-2 spike protein.

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in SARS-CoV-2 spike S2 ECD Recombinant protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SARS-CoV-2 spike protein S2 domain in SARS-CoV-2 spike S2 ECD Recombinant protein.

Tested Applications

ELISA Analysis: A representative lot detected SARS-CoV-2 spike protein S2 domain in ELISA applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).

Neutralizing: A representative lot neutralized SARS-CoV-2 spike protein S2 in Neutralizing applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SARS-CoV-2 spike protein S2 domain, Cat. No. ABF1063, is a rabbit polyclonal antibody that detects the S2 domain of SARS-CoV-2 spike protein and is tested for use in ELISA, Neutralization, and Western Blotting.

Postać fizyczna

Rabbit polyclonal antibody in serum without preservatives.

Przechowywanie i stabilność

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.

Nie możesz znaleźć właściwego produktu?  

Wypróbuj nasz Narzędzie selektora produktów.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej