ABE991

Anti-NeuroD1 Antibody

serum, from rabbit

• Synonim(y): Neurogenic differentiation factor 1, NeuroD1, Beta-cell E-box transcriptional activator 2, Beta2
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: serum
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• reaktywność gatunkowa: rat, mouse
• metody: ChIP: suitable; immunohistochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NP_035024
• numer dostępu UniProt: Q60867
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: mouse ... Neurod1(18012) ; rat ... Neurod1(29458)

Opis

Opis ogólny

NeuroD1 is a transcriptional activator that associates with p300 transcription co-activator protein to promote the expression of many important differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. NeuroD1 is required for amacrine cell fate as well as dendrite morphogenesis and the maintenance of cerebellar cortex. Also via its associate with chromatin, NeuroD1 helps regulate the expression of many other key transcriptional regulators of neurogenesis as well. NeuroD1 is expressed in developing brain and pancreas as well as in differentiating neurons in both the CNS and PNS systems. Disruption of NeuroD1 leads to early death in mice with several developmental and functional defects throughout the body pointing to this factors key role in development in many systems.

Immunogen

GST-tagged recombinant protein corresponding to mouse NeuroD1.

Zastosowanie

Immunohistochemistry Analysis: A representative lot detected NeuroD1 in developing pituitary tissue (Lavoie, P., et al. (2008). Molecular Endocrinology. 22:1647-1657).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated NeuroD1 in AtT-20 cell lysate (Langlais, D., et al. (2011). Molecular Endocrinology. 25:348-359).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-NeuroD1 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and Immunohistochemistry for the detection of NeuroD1.

Jakość

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NeuroD1 in 10 µg of rat brain tissue lysate.

Opis wartości docelowych

~40 kDa observed. Uncharacterized band(s) may appear in some lysates.

Postać fizyczna

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1