ABE215

Anti-Histone Macro H2A.1 Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Core histone macro-H2A.1, Histone macroH2A1.2, mH2A1, H2A.y, H2A/y
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human, mouse
• metody: immunocytochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NP_001152985.1
• numer dostępu UniProt: Q9QZQ8
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... H2AFJ(55766)

Opis

Opis ogólny

Histone MacroH2A (mH2A) is a variant histone also known as H2AFY. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. This histone variant is known to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. In contrast to most histone variants containing only subtle changes, mH2A contains a large non-histone domain (the macro domain) on its C-terminus, resulting in a histone approximately three times the size of conventional H2A. There are 3 isoforms of mH2A, two of which (mH2A1.1, mH2A1.2 ) are formed by alternative splicing and another (mH2A2) from a separate gene. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of posttranslational modifications throughout the cell cycle.

Specyficzność

This antibody recognizes Histone MacroH2A.1 at the macro domain.

Immunogen

Epitope: Macro domain

GST-tagged recombinant protein corresponding to mcro domain of mouse Histone MacroH2A.1.

Zastosowanie

Anti-Histone Macro H2A.1 Antibody is a Rabbit Polyclonal Antibody for detection of Histone Macro H2A.1 also known as H2A histone family member Y, histone macroH2A1.2 & has been validated in WB, ICC.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone MacroH2A.1 in NIH/3T3, A431, and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Jakość

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 0.2 µg/mL of this antibody detected histone MacroH2A.1 on 10 µg of HeLa acid extract.

Opis wartości docelowych

~ 40 kDa observed

Powiązanie

Replaces: 07-219

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HeLa acid extract

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable