ABE1943
Anti-HOXB13 Antibody
from rabbit, purified by affinity chromatography
Synonim(y):
Homeobox protein Hox-B13, HOXB13
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
human
metody
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... HOXB13(10481)
Opis ogólny
Homeobox protein Hox-B13 (UniProt Q92826) is encoded by the HOXB13 (also known as PSGD) gene (Gene ID 10481) in human. HOXB13 is a homeobox gene family member whose expression is restricted to prostate tissues and is highly correlated with that of androgen receptor (AR). Hox-B13 suppresses androgen-stimulated AR activity via direct AR interaction and thereby inhibits androgen-dependent (AD) prostate cancer (PCa) growth under androgen-rich conditions. On the other hand, HOXB13 is also known to be over-expressed in castration-resistant PCa (CRPC), where it plays a dichotomic role in promoting androgen-independent (AI) PCa growth. The zinc level in human prostate is markedly diminished in prostatic epithelial tumors and continues to decline during progression towards AI growth. HOXB13 over-expression upregulates transcription of its target genes SLC30A10 (ZnT10) and SLC30A4 (ZnT4) for zinc output transporters, which are responsible for the reduction in zinc level in CRPC. The diminished intracellular zinc level in turn stimulates NF-κB signaling and promotes invasion and metastasis of CRPC cells.
Specyficzność
This polyclonal antibody selectively reacts with human HOXB13, but not the homologous HOXA13 or HOXD13.
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of human HOXB13.
Zastosowanie
This Anti-HOXB13 Antibody is validated for use in Western Blotting, Immunocytochemistry and Immunohistochemistry for the detection of HOXB13.
Western Blotting Analysis: A representative lot detected HOXB13 expression in human prostate cancer cell lines MDA PCa 2b, LNCaP, and C4-2, but not in prostate cancer DU145, PC3, CWR22Rv-1, or the non-tumorigenic prostate epithelial line P69 (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124).
Immunocytochemistry Analysis: A representative lot detected HOXB13 nuclear expression in MDA PCa 2b and LNCaP, but not PC3 or DU145, human prostate cancer cell lines (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124).
Immunohistochemistry Analysis: A representative lot detected differential HOXB13 expressions among various androgen-dependent (AD) and androgen-independent (AI) human prostate cancer tissues (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124; Kim, Y.R., et. al. (2014). Oncogene. 33(37):4558-4567).
Immunocytochemistry Analysis: A representative lot detected HOXB13 nuclear expression in MDA PCa 2b and LNCaP, but not PC3 or DU145, human prostate cancer cell lines (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124).
Immunohistochemistry Analysis: A representative lot detected differential HOXB13 expressions among various androgen-dependent (AD) and androgen-independent (AI) human prostate cancer tissues (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124; Kim, Y.R., et. al. (2014). Oncogene. 33(37):4558-4567).
Jakość
Evaluated by Western Blotting in LNCaP cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected HOXB13 in 10 µg of LNCaP cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected HOXB13 in 10 µg of LNCaP cell lysate.
Opis wartości docelowych
~35 kDa observed
Postać fizyczna
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-HCI, pH 8.0, and 0.1% soidum azide.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
HOXB13 promotes androgen independent growth of LNCaP prostate cancer cells by the activation of E2F signaling.
Kim, YR; Oh, KJ; Park, RY; Xuan, NT; Kang, TW; Kwon, DD; Choi, C; Kim, MS; Nam, KI; Ahn, KY; Jung, C
Molecular Cancer null
Y-R Kim et al.
Oncogene, 33(37), 4558-4567 (2013-10-08)
Characteristically, prostate cancer (PCa) cells exhibit marked decrease in intracellular zinc; however, the mechanism responsible is not clearly understood. HOXB13 is involved in PCa progression and is overexpressed in castration-resistant PCa. DNA microarray analysis of LNCaP Pca cells showed that
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