ABE1877M

Anti-PAXIP1

from rabbit

• Synonim(y): PAX-interacting protein 1, PAX transactivation activation domain-interacting protein, PAXIP
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• reaktywność gatunkowa: human, mouse
• opakowanie: antibody small pack of 25 μL
• metody: ChIP: suitable (ChIP-seq); immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG
• numer dostępu NCBI: NP_061366
• numer dostępu UniProt: Q6NZQ4
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... PAXIP1(22976) ; mouse ... Paxip1(55982)

Opis

Opis ogólny

PAX-interacting protein 1 (UniProt: Q6NZQ4; also known as PAX transactivation activation domain-interacting protein, PAXIP1) is encoded by the Paxip1 (also known as Ptip) gene (Gene ID: 55982) in murine species. PAXIP1 is a nuclear matrix protein that is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes such as the MLL2/MLL3 complex. In vitro it is shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks. It recruits Tp53bp1 to DNA damage foci and effective DNA damage response appears to require the association with Tp53bp1 phosphorylated by ATM. It also recruits PAGR1A (Pax-Interacting Protein 1-associated glutamate rich protein 1a) to DNA damage sites and PAGR1A:PAXIP1 complex is believed to be essential for cell survival. PAXIP1 expression is reported in all tissues and high expression is observed in embryonic kidney and brain. PAXIP1 contains 6 BRCT domains and BRCT domains 1 and 2 mediate its interaction with PAGR1A and interaction with MLL2/MLL3 is accomplished with BRCT domains 5 and 6. Mice with defective Paxip1 gene are developmentally retarded and usually face embryonic lethality by day 9.5 (E9.5).

Specyficzność

This rabbit polyclonal antibody detects PAX-interacting protein 1 in human and murine cells. It targets an epitope within 199 amino acids from the N-terminal region.

Immunogen

Epitope: unknown

His-tagged recombinant fragment corresponding to 199 amino acids from the N-terminal region of murine PAX-interacting protein 1.

Zastosowanie

Anti-PAXIP1, Cat. No. ABE1877, is a highly specific rabbit polyclonal antibody that targets PAX-interacting protein 1 and has been tested for use in ChIP-seq and Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

Chromatin Immunoprecipitation Analysis: A representative lot detected PAXIP1 in mouse embryonic fibroblasts (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).

ChIP-seq Analysis: Analysis: A representative lot detected PAXIP1 in ChIP-seq applications (Callen, E., et. al. (2012). Immunity. 37(6):971-85).

Immunoprecipitation Analysis: A representative lot detected PAXIP1 in Immunoprecipitation applications (Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).

Immunoprecipitation Analysis: 1 µg from a representative lot detected PAXIP1 in nuclear extract of wild type and PTIP KO mouse embryonic fibroblasts (Courtesy of Dr. Ji-Eun Lee and Dr. Kai Ge at NIH).

Western Blotting Analysis: A representative lot detected PAXIP1 in Western Blotting applications (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).

Research Category
Epigenetics & Nuclear Function

Jakość

Evaluated by Western Blotting with with recombinant Pax-interacting protein 1.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant PAX-interacting protein 1.

Opis wartości docelowych

~51 kDa observed (recombinant); 119.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1