ABE1408

Anti-DNA polymerase beta Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): DNA polymerase beta, DNA polymerase beta
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: rat, human, mouse
• reaktywność gatunkowa (przewidywana na podstawie homologii): canine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), feline (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• metody: immunohistochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NP_002681
• numer dostępu UniProt: P06746
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: bovine ... Polb(614688) ; cat ... Polb(101099797) ; dog ... Polb(494001) ; frog ... Polb(448741) ; human ... POLB(5423) ; mouse ... Polb(18970) ; rat ... Polb(29240) ; rhesus monkey ... Polb(704801)

Opis

Opis ogólny

DNA polymerase beta (EC 2.7.7.7; UniProt P06746; also known as DNA pol beta, DNA polymerase beta subunit) is encoded by the POLB gene (Gene ID 5423) in human. DNA polymerase beta is involved in DNA recombination and base-excision repair (BER). Upon oxidative DNA damage, Polb translocates to the nucleus, where it associates with AP endonuclease 1 (Apex) to carry out DNA repair. Apex makes a single nick 5′ to an abasic site in double-stranded DNA, Polb then inserts the replacement nucleotide(s). Aberrant POLB overexpression results in an increased rate of spontaneous mutagenesis and a highly mutagenic tolerance phenotype. Thirty percent of all tumors are reported to harbor mutations in the POLB gene.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human DNA polymerase beta.

Zastosowanie

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DNA polymerase beta in normal rat testis, normal human kidney, as well as human lung & prostate cancer tissue sections.

This Anti-DNA polymerase beta Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DNA polymerase beta.

Jakość

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DNA polymerase beta in 10 µg of human and mouse testis tissue, Jurkat, and HeLa lysates.

Opis wartości docelowych

~38 kDa observed. Uncharacterized band(s) may appear in some lysates.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable