ABE1407

Anti-Ubiquitin-conjugating enzyme E2 A Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Ubiquitin-conjugating enzyme E2 A, RAD6 homolog A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: mouse, rat, human
• reaktywność gatunkowa (przewidywana na podstawie homologii): primate (based on 100% sequence homology), sheep (based on 100% sequence homology), horse (based on 100% sequence homology), chicken (based on 100% sequence homology)
• metody: western blot: suitable
• numer dostępu NCBI: NP_003327
• numer dostępu UniProt: P49459
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... UBE2A(7319)

Opis

Opis ogólny

Ubiquitin-conjugating enzyme E2 A (EC 6.3.2.19; UniProt P49459; also known as hHR6A, HR6A, RAD6 homolog A, Ubiquitin carrier protein A) is encoded by the UBE2A (also known as MRXS30, MRXSN, RAD6A, UBC2) gene (Gene ID 7319) in human. UBE2A accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2A is involved in transcription regulation by catalyzing the monoubiquitination of histone H2B Lys120 to form H2BK120ub1. H2BK120ub1 serves as a specific signal for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, as well as a prerequisite for H3K4me and H3K79me formation. In vitro, UBE2A catalyzes both Lys11 and Lys48-linked polyubiquitination. UBE2A is also involved in UV-damaged DNA repair and aberrant UBE2A can cause mental retardation syndromic X-linked Nascimento-type (MRXSN).

Specyficzność

Expected to react with all three spliced isoforms.

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human Ubiquitin-conjugating enzyme E2 A sequence near the C-terminus.

Zastosowanie

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Ubiquitin-conjugating enzyme E2 A Antibody is validated for use in Western Blotting for the detection of Ubiquitin-conjugating enzyme E2 A.

Jakość

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ubiquitin-conjugating enzyme E2 A in 10 µg of HeLa cell lysate.

Opis wartości docelowych

~17 kDa observed. Uncharacterized band(s) may appear in some lysates.

Postać fizyczna

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable