ABE103

Anti-SIRT7 Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): NAD-dependent deacetylase sirtuin-7, SIR2-like protein 7
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klon: polyclonal
• application: IHC; WB
• reaktywność gatunkowa: rat, human
• metody: immunohistochemistry: suitable; western blot: suitable
• citations: 2

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: rat, human
• metody: immunohistochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NP_057622.1
• numer dostępu UniProt: Q9NRC8
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SIRT7(51547)

Opis

Opis ogólny

The silent information regulator (SIR) genes (sirtuins) are a highly conserved family of proteins, which contain one or more sirtuins present in various species from bacteria to mammals. There are seven sirtuin genes that encode seven sirtuin enzymes, SIRT1 through SIRT7, all of which are dependent on oxidized nicotinamide adenine dinucleotide (NAD+). These proteins serve as mono-ADP-ribosyltransferases and deacetylate histones as a means of silencing specific chromatin regions. Further research is still required to elicidate the link between its effects on cellular metabolism, stress resistance, and cellular proliferation and its proposed role in mediating general lifespan. It is reported that Sirt7 expression demonstrates an anti-proliferative role with tumorigenic potential, in several murine cell lines.

Specyficzność

This antibody recognizes the N-terminus of the expressed protein of SIRT7.

Immunogen

Epitope: N-terminus.

KLH-conjugated linear peptide corresponding to the N-terminus of the expressed protein of human SIRT7.

Zastosowanie

Immunohistochemistry Analysis: A 1:00 dilution from a representative lot detected the expressed protein of SIRT7 in human liver tissue lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Use Anti-SIRT7 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect SIRT7 also known as NAD-dependent deacetylase sirtuin-7, SIR2-like protein 7.

Jakość

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected the expressed protein of SIRT7 in 10 µg of human liver tissue lysate.

Opis wartości docelowych

~45 kDa observed. An additional isoform at ~20 kDa is known to exist. An uncharacterized band at ~30 kDa has been observed in some lysates.

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Human liver tissue lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable