ABC974
Anti-ATG8 Antibody
from rabbit, purified by affinity chromatography
Synonim(y):
Atg8, Atg8a, Atg8b, Autophagy-specific gene 8a, Autophagy-specific gene 8b
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
Polecane produkty
pochodzenie biologiczne
rabbit
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
Drosophila
reaktywność gatunkowa (przewidywana na podstawie homologii)
Caenorhabditis elegans (based on 100% sequence homology)
metody
western blot: suitable
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
fruit fly ... Atg8A(32001)
Opis ogólny
Autophagy-specific gene 8a and 8b (UniProt Q9W2S2 and Q9VEG5, respectively) are encoded by the drosophila Atg8a (ORF Dmel_CG32672; Gene ID 32001) and Atg8b (ORF Dmel_CG12334; Gene ID 42132) gene, respectively. Autophagy is a fundamental an adaptive response to cellular stress caused by, for example, starvation and viral infection. Autophagy is a process conserved from yeast to human and involves the formation of double-membrane vesicles for the purpose of recycling nutrients by means of degrading the engulfed content. In resting cells, the majority of Atg8 is in an unlipidated form called Atg8-I. During autophagy, Atg8-I is conjugated with a lipid moiety, and the lipidated form (Atg8-II) is recruited to autophagosomal membranes. An elevated Atg8-II level therefore serves as an indicator of autophagy activation. LC3 is the mammalian ortholog of drosophila Atg8.
Specyficzność
Reacts with the unlipidated (Atg8-I) and lipidated (Atg8-II) forms of drosophila Atg8a and Atg8b (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Immunogen
Epitope: Near N-terminus.
Linear peptide based on drosophila Atg8a and Atg8b sequences near the N-terminus.
Zastosowanie
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-ATG8 Antibody is validated for use in Western Blotting for the detection of ATG8.
Western Blotting Analysis: A representative lot detected the induction of the lipidated Atg8-II band in wild-type flies upon Rift Valley fever virus (RVFV) infection, while an enhanced Atg8-II induction was observed in Toll-1-overexpressing flies and no Atg8-II induction by RVFV infection was seen among Toll-7 mutant flies (Moy, R.H., et al. (2014). Immunity. 40(1):51-65).
Western Blotting Analysis: A representative lot detected sdRNA-targeted downregulation of Atg8a and Atg8b in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Western Blotting Analysis: A representative lot detected the 16 kDa Atg8-I band (unlipidated) as well as the serum starvation- and vesicular stomatitis virus (VSV) infection-induced 14 kDa Atg8-II band (lipidated) in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Western Blotting Analysis: A representative lot detected sdRNA-targeted downregulation of Atg8a and Atg8b in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Western Blotting Analysis: A representative lot detected the 16 kDa Atg8-I band (unlipidated) as well as the serum starvation- and vesicular stomatitis virus (VSV) infection-induced 14 kDa Atg8-II band (lipidated) in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
Jakość
Evaluated by Western Blotting in DL1 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATG8 in 10 µg of DL1 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATG8 in 10 µg of DL1 cell lysate.
Opis wartości docelowych
~16/14 kDa observed. The upper band of the doublet represents the unlipidated Atg8-I form, while the lower band of the doublet represents the lipidated Atg8-II form that is upregulated upon autophagy induction.
Postać fizyczna
Affinity purified
Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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