ABC118

Anti-phospho Beclin Antibody (Thr119)

from rabbit, purified by affinity chromatography

• Synonim(y): Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology)
• metody: inhibition assay: suitable (peptide); western blot: suitable
• numer dostępu NCBI: NP_003757
• numer dostępu UniProt: Q14457
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pThr119)
• informacje o genach: human ... BECN1(8678)

Opis

Opis ogólny

The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson′s and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.

Specyficzność

This antibody recognizes the BH3 domain of Beclin phosphorylated at Thr119.

Immunogen

Epitope: BH3 domain

KLH-conjugated linear peptide corresponding to the BH3 domain of human Beclin phosphorylated at Thr119.

Zastosowanie

Detect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting/Peptide Inhibition Analysis: 5 µg/mL from a representative lot detected phospho Beclin (Thr119) in LNCap cell lysate. Peptide inhibition was observed with the addition of phospho peptides, but not with the addition of non-phospho peptides.

Jakość

Evaluated by Western Blotting in human prostate tumor tissue lysate.

Western Blotting Analysis: 5 µg/mL of this antibody detected 10 µg of human prostate tumor tissue lysate.

Opis wartości docelowych

~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Postać fizyczna

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Human prostate tumor tissue lysate.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable