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Key Documents

AB5935

Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40

Chemicon®, from rabbit

Synonim(y):

Tyrosine Monooxygenase

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

oczyszczone przez

affinity chromatography

reaktywność gatunkowa

avian, rat, mouse

reaktywność gatunkowa (przewidywana na podstawie homologii)

quail (100% sequence homology), nonhuman primates (100% sequence homology), human (100% sequence homology), porcine (100% sequence homology)

producent / nazwa handlowa

Chemicon®

metody

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

phosphorylation (pSer40)

informacje o genach

human ... TH(7054)
rat ... Th(25085)

Opis ogólny

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Specyficzność

Other species have not been tested.
Tyrosine Hydroxylase, phosphoSer40. The antibody recognizes a protein of 60 kDa corresponding to TH phosphorylated at Ser40. Depending on brain region being studied, protein loads and detection methods used some higher molecular weight bands may be detected.

Immunogen

Epitope: phospho-Ser40
Synthetic peptide from the phosphoSer40 of rat Tyrosine Hydroxylase.

Zastosowanie

Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40 detects level of Tyrosine Hydroxylase & has been published & validated for use in IC, IH & WB.
Immunohistochemistry:
A 1:1,000 dilution of a previous lot was used in IH.

Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in IC.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Jakość

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected TYROSINE HYDROX PSER40 on 10 μg of PC12 lysates.

Opis wartości docelowych

60 kDa

Postać fizyczna

ImmunoAffinity Purified
Purified rabbit polyclonal liquid in buffer containing 10mM Hepes, pH 7.5, 150mM NaCl, 100 µg/mL BSA, and 50% glycerol.

Przechowywanie i stabilność

Stable for 6 months at -20°C in undiluted from date of receipt.

Komentarz do analizy

Control
POSITIVE CONTROL: brain (sympathetic nerve terminals) and adrenal glands.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Phosphodiesterase 4 inhibition enhances the dopamine D1 receptor/PKA/DARPP-32 signaling cascade in frontal cortex.
Mahomi Kuroiwa,Gretchen L Snyder,Takahide Shuto,Atsuo Fukuda,Yuchio Yanagawa et al.
Psychopharmacology (Psychopharmacologia) null
Zhenqiang Zhao et al.
Frontiers in cellular neuroscience, 10, 291-291 (2017-01-10)
Mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs) are used for the culture of human embryonic stem cells (hESCs). MEFs and HFFs differed in their capacity to support the proliferation and pluripotency of hESCs and could affect cardiac differentiation
Regulation of serine (Ser)-31 and Ser40 tyrosine hydroxylase phosphorylation during morphine withdrawal in the hypothalamic paraventricular nucleus and nucleus tractus solitarius-A2 cell group: role of ERK1/2.
Cristina Nu?ez, M Luisa Laorden, M Victoria Milanes
Endocrinology null
Restricted role of CRF1 receptor for the activity of brainstem catecholaminergic neurons in the negative state of morphine withdrawal.
Juan-Antonio Garcia-Carmona,Pilar Almela,Alberto Baroja-Mazo,M Victoria Milanes,M Luisa Laorden
Psychopharmacology (Psychopharmacologia) null
Distinct roles of PDE4 and PDE10A in the regulation of cAMP/PKA signaling in the striatum.
Nishi, A; Kuroiwa, M; Miller, DB; O'Callaghan, JP; Bateup, HS; Shuto, T; Sotogaku et al.
The Journal of Neuroscience null

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