AB5154
Anti-Cav2.2 Antibody
Chemicon®, from rabbit
Synonim(y):
Voltage Gated Calcium Channel (N-type) alpha 1B Subunit, Cacna1b
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
rat
producent / nazwa handlowa
Chemicon®
metody
immunocytochemistry: suitable
immunohistochemistry: suitable (tissue)
immunoprecipitation (IP): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
rat ... Cacna1B(257648)
Specyficzność
Recognizes a 1B subunit (both LMW and HMW forms) of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far.
SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested.
SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested.
Immunogen
Purified peptide CNB1 from 851-867 of alpha 1B subunit of rat brain voltage-gated calcium channel (Accession Q02294; Westenbroek et al. 1992) containing N-terminal lysine and tyrosine, conjugated to KLH with gluteraldehyde (Harlow & Lane 1988).
Zastosowanie
Detect Cav2.2 using this Anti-Cav2.2 Antibody validated for use in IC, IP & WB.
In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Immunoprecipitation
Immunocytochemistry. Recommend subsequent staining with a labeled second antibody.
Western blot: 1.5 to 3.0 μg/mL (1:100-1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunoprecipitation
Immunocytochemistry. Recommend subsequent staining with a labeled second antibody.
Western blot: 1.5 to 3.0 μg/mL (1:100-1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Neuroinflammation & Pain
Ion Channels & Transporters
Neuroinflammation & Pain
Postać fizyczna
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Przechowywanie i stabilność
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at 2-8°C in undiluted aliquots for up to 2 weeks. For longer periods, it is recommended to add glycerol (ACS grade or better) at a ratio of 1:1 and store the antibody at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Komentarz do analizy
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
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Klasa zagrożenia wodnego (WGK)
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R E Westenbroek et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 12(6), 2259-2267 (1992-06-01)
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