AB1594P
Anti-Serotonin Transporter Antibody
Chemicon®, from rabbit
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
rat, human
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... HTT(3064) , SLC6A4(6532)
Specyficzność
Serotonin Transporter (SERT). An antibody raised against a similar peptide has been shown to react with a SERT in baculovirus cells expressing recombinant rat and human SERT. Antibody activity may be reduced considerably when detecting endogenous SERT from rat or human cells. SERT has been observed to migrate as 94 kDa (rat platelet), 80 kDa (pulmonary membranes) and 76 kDa (rat brain). This could be due to differences in postranslational membrane modifications (e.g. glycosylation).
Immunogen
A 15 amino acid sequence between transmembrane domain 7 and 8 of the rat serotonin transporter. This region is presumably from the extracellular domain.
Zastosowanie
Anti-Serotonin Transporter Antibody is an antibody against Serotonin Transporter for use in ELISA & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Neuronal & Glial Markers
Ion Channels & Transporters
Neuronal & Glial Markers
Western blot: 1-10 μg/mL
Cell pellets are resuspended in 100 μL lysis buffer (25 mM Tris, pH 7.6, 150 mM NaCl, 1 μg/mL aprotonin, 10 μg/mL leupeptin, 1 mM phenylmethylsulfonylfluoride, 1 mM sodium orthovanadate, 1 mM EDTA, and 1% Triton X-100). Samples were incubated for 1 hour at 4°C and were pelleted at high speed for 10 minutes at 4°C, and 100 μg protein per sample was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
ELISA: 1:100,000 primary EIA using immunogen only (50-100 ng SERT peptide (Catalog Number AG297)/well). Not tested on purified protein at the stated dilutions.
Optimal working dilutions must be determined by end user.
Cell pellets are resuspended in 100 μL lysis buffer (25 mM Tris, pH 7.6, 150 mM NaCl, 1 μg/mL aprotonin, 10 μg/mL leupeptin, 1 mM phenylmethylsulfonylfluoride, 1 mM sodium orthovanadate, 1 mM EDTA, and 1% Triton X-100). Samples were incubated for 1 hour at 4°C and were pelleted at high speed for 10 minutes at 4°C, and 100 μg protein per sample was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
ELISA: 1:100,000 primary EIA using immunogen only (50-100 ng SERT peptide (Catalog Number AG297)/well). Not tested on purified protein at the stated dilutions.
Optimal working dilutions must be determined by end user.
Postać fizyczna
Affinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide.
Przechowywanie i stabilność
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
The cellular distribution of serotonin transporter is impeded on serotonin-altered vimentin network.
Ahmed, BA; Bukhari, IA; Jeffus, BC; Harney, JT; Thyparambil, S; Ziu, E; Fraer, M; Rusch et al.
Testing null
Expression of the serotonin transporter SERT in the genital tract of cattle.
Luigi M Pavone, Simona Tafuri, Roberta Lo Muto, Anna Spina, Alla E Lychkova et al.
Regulatory Peptides null
Christophe Wersinger et al.
The European journal of neuroscience, 24(1), 55-64 (2006-08-03)
alpha-Synuclein (alpha-Syn), a protein primarily localized in the presynaptic compartment of neurons, is known to regulate dopaminergic neurotransmission by negatively modulating dopamine transporter activity and regulating its trafficking to or away from the cell surface. Given the considerable homology between
A M Thompson et al.
Developmental neuroscience, 27(1), 1-12 (2005-05-12)
To investigate the putative role of serotonin (5-HT) in auditory brainstem development, the expression of the 5-HT transporter (5-HTT) was evaluated in the normal mouse brainstem at 6 different postnatal ages. The brains of C3H/HeJ mice at birth (P0) and
Fate map of serotonin transporter-expressing cells in developing mouse thyroid.
Spina A, Rea S, De Pasquale V, Mastellone V, Avallone L, Pavone LM.
Anatomical Record (Hoboken, N.J. : 2007) (2007)
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