AB1582
Anti-Choline Acetyltransferase Antibody
CHEMICON®, sheep polyclonal
Synonim(y):
ChAT
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
product name
Anti-Choline Acetyltransferase Antibody, serum, Chemicon®
pochodzenie biologiczne
sheep
Poziom jakości
forma przeciwciała
serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
rat, guinea pig, rabbit
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
rabbit ... Chat(100342226)
rat ... Chat(290567)
Specyficzność
Choline Acetyltransferase (ChAT). Will stain cholinergic neurons in rat, guinea-pig and rabbit central and peripheral nervous systems.
Immunogen
A 22 amino acid peptide from porcine ChAT, coupled to KLH. The sequence is H-GLFSSYRLPGHTQDTLVAQKSS-NH2.
Zastosowanie
Immunohistochemistry: 1:1,000-1:2,000
Immunoblotting
Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582
STAINING OF GUINEA PIG ILEUM
1) Pin the ileum out flat and tight.
2) Fix overnight at 4°C in modified Zamboni′s (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).
3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.
4) Dissect off ALL circular muscle.
5) Preincubate with 10% normal donkey serum.
6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.
7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.
IMPORTANT NOTE: It is essential to remove ALL circular muscle.
Immunoblotting
Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582
STAINING OF GUINEA PIG ILEUM
1) Pin the ileum out flat and tight.
2) Fix overnight at 4°C in modified Zamboni′s (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).
3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.
4) Dissect off ALL circular muscle.
5) Preincubate with 10% normal donkey serum.
6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.
7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.
IMPORTANT NOTE: It is essential to remove ALL circular muscle.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neurotransmitters & Receptors
Neuronal & Glial Markers
This Anti-Choline Acetyltransferase Antibody is validated for use in IH, WB for the detection of Choline Acetyltransferase.
Postać fizyczna
Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.
Przechowywanie i stabilność
Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
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Kod klasy składowania
10 - Combustible liquids
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Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Immunohistochemical localization of calbindin-D28k and calretinin in the brainstem of anuran and urodele amphibians.
Ruth Morona, Agustin Gonzalez, Ruth Morona, Agustin Gonzalez, Ruth Morona, Agustin Gonzalez
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I Robert et al.
Journal of molecular neuroscience : MN, 11(3), 243-251 (1999-05-27)
cDNA containing the entire coding region of the human choline acetyltransferase gene (hChAT) was fused to the influenza virus hemagglutinin (HA) epitope preceded by a Kozak sequence. The recombinant HA-hChAT was then inserted into an expression vector under the transcriptional
Immuno-localization of vesicular acetylcholine transporter in mouse taste cells and adjacent nerve fibers: indication of acetylcholine release.
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J L Morris, B S Zhu, I L Gibbins, W W Blessing
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