AB1542
Anti-Tyrosine Hydroxylase Antibody
CHEMICON®, sheep polyclonal
Synonim(y):
Tyrosine 3-hydroxylase, Tyrosine 3-hydroxylase, tyrosine hydroxylase
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
Nazwa produktu
Anti-Tyrosine Hydroxylase Antibody, Chemicon®, from sheep
pochodzenie biologiczne
sheep
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
rat, mouse
reaktywność gatunkowa (przewidywana na podstawie homologii)
mammals
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... TH(7054)
Opis ogólny
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Specyficzność
Tyrosine Hydroxylase (TH, Tyrosine Monooxygenase). Cross-reacts with all mammalian forms tested to date and some non-mammalian forms.
Immunogen
Native tyrosine hydroxylase from rat pheochromocytoma (J. Biol. Chem.,1982, 257:9416-9423).
Zastosowanie
Anti-Tyrosine Hydroxylase Antibody is an antibody against Tyrosine Hydroxylase for use in IH & WB.
Immunohistochemistry(peroxidase): A previous lot of this antibody was used at 1:1,000 dilution. The antibody gives specific labeling of noradrenergic axons in primate cerebral cortex (Brain Res., 1989, 500:313-324.).
Note: It is important that you use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.
Optimal working dilutions must be determined by the end user.
Note: It is important that you use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neurotransmitters & Receptors
Neuronal & Glial Markers
Jakość
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of mouse brain lysates.
Opis wartości docelowych
~60 kDa
Postać fizyczna
ImmunoAffinity Purified
Purified sheep polyclonal in buffer containing 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Przechowywanie i stabilność
Stable for 6 months at -20 to -80°C in undiluted aliquots from date of receipt. Store in a NON-self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Komentarz do analizy
Control
Positive: brain (corpus striatum) and adrenal gland. Negative: liver.
Positive: brain (corpus striatum) and adrenal gland. Negative: liver.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Certyfikaty analizy (CoA)
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