71194-M
0.5 M THP Solution
Reducing agent compatible with His•Bind chromatography
Synonim(y):
Tris(3-hydroxypropyl)phosphine
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About This Item
Kod UNSPSC:
41106511
NACRES:
NA.77
Polecane produkty
Poziom jakości
Postać
solution
producent / nazwa handlowa
Novagen®
warunki przechowywania
OK to freeze
metody
protein purification: suitable
Zastosowanie
sample preparation
temp. przechowywania
−20°C
Opis ogólny
Reducing agent compatible with HIS-BIND® chromatography
THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT). Against oxidized glutathione, the reducing capacity of THP (0.232 meq/g) is greater than that of TCEP (0.182 meq/g). THP is also more resistant to air oxidation than DTT and is compatible with immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM. The common working concentration of THP for many applications is 15 mM. THP is provided as a ready-to-use 0.5 M solution in water.
Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.
Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.
Cechy i korzyści
Compatability with common methods for purifying His-tagged proteins - immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM
Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)
Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione
Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage
Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)
Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione
Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage
Ostrzeżenie
Toxicity: Corrosive (L)
Inne uwagi
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Informacje prawne
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Dam. 1 - Skin Corr. 1B
Kod klasy składowania
8B - Non-combustible corrosive hazardous materials
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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