616394

TPEN

• Synonim(y): TPEN, N,N,Nʹ,Nʹ- tetrakis-(2-Pyridylmethyl)ethylenediamine
• Wzór empiryczny (zapis Hilla): C₂₆H₂₈N₆
• Numer CAS: 16858-02-9
• Masa cząsteczkowa: 424.54
• Numer MDL: MFCD00036918
• Kod UNSPSC: 12352116
• NACRES: NA.25

Właściwości

• Próba: ≥97% (titration)
• Poziom jakości: 100
• Postać: solid
• przydatność reakcji: reagent type: chelator
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• kolor: tan
• rozpuszczalność: ethanol: 10 mg/mL; 0.1 M phosphate pH 7.0: 25 μM; DMSO: soluble
• Warunki transportu: ambient
• temp. przechowywania: 15-25°C
• InChI: 1S/C26H28N6/c1-5-13-27-23(9-1)19-31(20-24-10-2-6-14-28-24)17-18-32(21-25-11-3-7-15-29-25)22-26-12-4-8-16-30-26/h1-16H,17-22H2
• Klucz InChI: CVRXLMUYFMERMJ-UHFFFAOYSA-N

Opis

Opis ogólny

Lipid-soluble heavy metal chelator that crosses artificial and natural membranes. Auxiliary reagent in certain techniques used for measuring free Ca²⁺ in cells. Has an extremely high affinity for heavy metals but a low affinity for Ca²⁺ and Mg²⁺. Induces DNA fragmentation when chelated with Zn²⁺ and causes apoptosis in thymocytes. Strips heavy metals from liposome-trapped QUIN 2. Shown to be useful in extracting Aβ depostis from Alzheimer′s disease brain tissue.

Lipid-soluble heavy metal chelator that crosses artificial and natural membranes. Auxiliary reagent in certain techniques used for measuring free Ca²⁺ levels in cells. Also useful in extracting Aβ amyloid deposits from Alzheimer’s disease brain tissue. Has extremely high affinity for heavy metals but low affinity for Ca²⁺ and Mg²⁺. Induces DNA fragmentation when chelated with Zn²⁺ and induces apoptosis in thymocytes. Strips heavy metals from liposome-trapped QUIN 2 (Cat. No. 551826).

Zastosowanie

• Poly(ADP-ribosyl)ation of p53 contributes to TPEN-induced neuronal apoptosis.: This study investigates the role of poly(ADP-ribosyl)ation of the tumor suppressor protein p53 in neuronal apoptosis induced by TPEN, highlighting mechanisms of cell death in neurodegenerative diseases (Kim HL et al., 2015).


• Essential role of p53 in TPEN-induced neuronal apoptosis.: This article explores the critical involvement of the p53 protein in apoptosis initiated by TPEN in neuronal cells, offering insights into potential therapeutic targets for neuroprotection (Ra H et al., 2009).


• Clioquinol induces autophagy in cultured astrocytes and neurons by acting as a zinc ionophore.: Demonstrates how Clioquinol, similar to TPEN, functions as a zinc ionophore to induce autophagy, particularly in neurological contexts, which may be relevant for understanding TPEN′s mechanisms (Park MH et al., 2011).

Ostrzeżenie

Toxicity: Standard Handling (A)

Rekonstytucja

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Alternately, acidify (pH ≤3) with HCl for long term storage. Stock solutions are stable for up to 3 months at -20°C.

Inne uwagi

Fonte, J., et al. 2001. J. Alzheimer’s Dis. 3, 209.
Cherny, R.A., et al. 1999. J. Biol. Chem. 274, 23223.
Aballay, A., et al. 1995. Biochem. J.312, 919.
Jiang, S., et al. 1995. Lab. Invest.73, 111.
Sheridan, R.E. and Deshpande, S.S. 1995. Toxicon33, 539.
McCabe, M.J., et al. 1993. Lab. Invest. 69, 101.
Hinkle, P.M., et al. 1992. J. Biol. Chem.267, 25553.
Baba, A., et al. 1991. Brain Res. 557, 103.
Forbes, I.J., et al. 1991. Exp. Cell Res. 195, 224.
Csermely, P. and Somogyi, J. 1989. J. Cellular Physiol.138, 593.
Raspe, E., et al. 1989. Eur. J. Pharmacol.163, 345.
Arslan, L.P., et al. 1985. J. Biol. Chem.260, 2719.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable