567704

Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.

• Synonim(y): SCDase
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• pochodzenie biologiczne: bacterial (Pseudomonas spp.)
• Poziom jakości: 100
• Postać: liquid
• aktywność właściwa: ≥1 units/mL
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; avoid repeated freeze/thaw cycles
• obecność zanieczyszczeń: α- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases, none detected
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Sphingolipid ceramide N-deacylase (SCDase) is a native sphingolipid ceramide N-deacylase from Pseudomonas species. It hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in several sphingolipids and gangliosides. This enzyme catalyzes the condensation reaction between fatty acids and sphingosine bases and forms sphingolipids. It is also involved in transacylation reactions. SCDase is not recommended for hydrolysis of ceramide. It is also used to prepare lysoglycosphingolipids (lysoGSLs) effortlessly.

Zastosowanie

Sphingolipid ceramide N-Deacylase, Pseudomonas sp. has been used as a PP2A enhancer to study its effects on phosphatase and tensin homolog (PTEN) in forkhead box O3 (FOXO3a) phosphorylation in primary CD4⁺ T cells.

Ostrzeżenie

Toxicity: Standard Handling (A)

Definicja jednostki

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.

Postać fizyczna

In 50 mM sodium acetate buffer, 0.1% LUBROL PX Detergent, pH 6.0.

Rekonstytucja

Following initial thaw, aliquot and freeze (-20°C).

Inne uwagi

Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable