525295
PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20)
liquid, clone PY20, Calbiochem®
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
PY20, monoclonal
Postać
liquid
zawiera
≤0.1% sodium azide as preservative
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
avoid repeated freeze/thaw cycles
izotyp
IgG2b
Warunki transportu
wet ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
phosphorylation (pTyr)
informacje o genach
human ... PID1(55022)
Opis ogólny
Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.
Immunogen
KLH conjugated to phosphotyrosine, glycine, and alanine
Zastosowanie
ELISA (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Opakowanie
Please refer to vial label for lot-specific concentration.
Ostrzeżenie
Toxicity: Standard Handling (A)
Postać fizyczna
In 10 mM sodium phosphate, pH 7.4.
Rekonstytucja
Following initial thaw, aliquot and freeze (-20°C).
Komentarz do analizy
Positive Control
Any cell lysate or tissue extract
Any cell lysate or tissue extract
Inne uwagi
Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Dheeraj Soni et al.
American journal of physiology. Lung cellular and molecular physiology, 312(6), L1003-L1017 (2017-04-08)
Vascular endothelial protein tyrosine phosphatase (VE-PTP) stabilizes endothelial adherens junctions (AJs) through constitutive dephosphorylation of VE-cadherin. Here we investigated the role of stromal interaction molecule 1 (STIM1) activation of store-operated Ca2+ entry (SOCE) in regulating AJ assembly. We observed that
Wojciech Stec et al.
Oncotarget, 9(9), 8560-8572 (2018-03-02)
Despite intensive research no therapies targeted against the oncogenic EGFRvIII are present in the clinic. One of the reasons is the elusive nature of the molecular structure and activity of the truncated receptor. The recent publications indicate the EGF-bound wild-type
Sebastian Alvarado et al.
Nature communications, 6, 6513-6513 (2015-03-12)
Complex quantitative traits, like size and behaviour, are a pervasive feature of natural populations. Quantitative trait variation is the product of both genetic and environmental factors, yet little is known about the mechanisms through which their interaction generates this variation.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej