328000

ERK Activation Inhibitor Peptide I, Cell-Permeable

The ERK Activation Inhibitor Peptide I, Cell-Permeable controls the biological activity of ERK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonim(y): ERK Activation Inhibitor Peptide I, Cell-Permeable, Ste-MEK1₁₃, Ste-MPKKKPTPIQLNP-NH₂, Erk Inhibitor IV
• Wzór empiryczny (zapis Hilla): C₈₆H₁₅₃N₁₉O₁₇S
• Masa cząsteczkowa: 1757.32
• Kod UNSPSC: 12352200
• NACRES: NA.77

Właściwości

• Poziom jakości: 100
• Próba: ≥95% (HPLC)
• Formularz: lyophilized solid
• siła działania: 2.5 μM IC₅₀
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; desiccated (hygroscopic); protect from light
• kolor: white
• rozpuszczalność: water: 1 mg/mL; DMSO: 5 mg/mL
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

A cell-permeable and reversible stearated 13-amino acid peptide corresponding to the N-terminus of MEK1 (MAPKK) that acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of Elk1. Selectively binds to ERK2 and prevents its interaction with MEK (IC₅₀ = 2.5 µM). Potently blocks ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells (IC₅₀ = 13 µM). Does not affect the activation of JNKs or p38.

A cell-permeable, reversible, and stearated, 13-amino acid peptide corresponding to the N-terminus of MAPK/ERK kinase (MEK). Acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of ELK1. Selectively binds to ERK2 and prevents its interaction with MEK (IC₅₀ = 2.5 µM). Shown to block ERK activity in PMA-stimulated NIH3T3 cells and NGF-treated PC12 cells (IC₅₀ = 13 µM). Does not affect the activation of either JNKs or p38 MAP kinase.

Działania biochem./fizjol.

Cell permeable: yes

Primary Target
MEK

Product does not compete with ATP.

Reversible: yes

Opakowanie

Packaged under inert gas

Ostrzeżenie

Toxicity: Standard Handling (A)

Sekwencja

Ste-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂ (Ste = stearated)

Postać fizyczna

Supplied as a trifluoroacetate salt.

Rekonstytucja

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Inne uwagi

Kelemen, B.R., et al. 2002. J. Biol. Chem.277, 8741.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable