322327

Diphtheria Toxin, CRM Mutant

Diphtheria Toxin, CRM197 Mutant, CAS 92092-36-9, is a nontoxic material with a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity.

• Synonim(y): Diphtheria Toxin, CRM Mutant
• Numer CAS: 92092-36-9
• Kod UNSPSC: 12352202

Właściwości

• Poziom jakości: 100
• Postać: solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; avoid repeated freeze/thaw cycles
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

CRM₁₉₇ is a nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity. Does not catalyze the ADP ribosylation of EF-2 factor. Immunologically, the CRM₁₉₇ protein is identical to the wild-type toxin.

Cross-reactive material of diphtheria toxin (CRM₁₉₇). A nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A that results in complete loss of enzymatic activity. Inhibits smooth muscle cell proliferation in a dose-dependent manner. Immunologically, the CRM₁₉₇ is identical to the wild-type toxin and is useful as a carrier protein of conjugate vaccines.

Działania biochem./fizjol.

Cell permeable: no

Primary Target
Mutant with loss of enzymatic activity to catalyze the ADP ribosylation of EF-2 factor

Reversible: no

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

Lyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.

Rekonstytucja

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.

Please refer to vial label for lot-specific reconstitution volume.

Komentarz do analizy

One major band by SDS-PAGE

Inne uwagi

Kusano, I., et al. 2001. Cell Struct. Funct.26, 279.
Kaefer, M., et al. 2000. J. Urol.163, 580.
Gupta, R.K., et al. 1997. Vaccine15, 1341.
Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol.251, 175.
Giannini, G., et al. 1984. Nucleic Acids Res.12, 4063.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable