204888

Complement C5, Human

Complement C5, Human, CAS 80295-53-0. is a native glycoprotein composed of two non-identical subunits of M.W. 120 kDa (α) and 75 kDa (β) linked by disulfide bonds.

• Synonim(y): Complement C5, Human
• Numer CAS: 80295-53-0
• Numer MDL: MFCD00130840
• Kod UNSPSC: 12352202
• NACRES: NA.25

Właściwości

• Poziom jakości: 200
• Próba: ≥95% (SDS-PAGE)
• Postać: liquid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; avoid repeated freeze/thaw cycles
• zanieczyszczenia: IgG, IgA, IgM, albumin, ceruloplasmin or functionally C3, C6, C7, C8, or C9, trace
• Warunki transportu: wet ice
• temp. przechowywania: −70°C

Opis

Opis ogólny

Native, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120 kDa (α) and 75 kDa (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in the assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at peptide bond 74 resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form lytic C5b-9 complement membrane attack complex (MAC).

Native, human C5 complement component. Glycoprotein composed of two non-identical subunits of M.W. 120,000 (α) and 75,000 (β) linked by disulfide bonds. Present in normal human serum at 70 µg/ml. Activation of complement via either the classical or alternative pathway can result in assembly of C5-cleaving enzymes (C5 convertases) on target surfaces. Both C5 convertases cleave the C5 α-chain at the peptide bond 74, resulting in production of C5a (M.W. 11,200) and C5b fragments (M.W. 185,000). Released C5a peptide is one of the three complement-derived anaphylatoxins. C5b fragment combines with C6, C7, C8, and C9 to form the lytic C5b-9 complement membrane attack complex (MAC).

Działania biochem./fizjol.

≥100,000 C₅H₅0 units/mg; ≥60% of the C5 activity in normal human serum on a mg/mg basis

Opakowanie

Please refer to vial label for lot-specific concentration.

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

In PBS, pH 7.2.

Uwaga dotycząca przygotowania

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Rekonstytucja

Following initial thaw, aliquot and freeze (-70°C).

Inne uwagi

Janatova, J. 1988. Methods Enzymol. 162, 579.
Wetsel, R.A., et al. 1980. J. Immunol. Methods 35, 319.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable