196810

Peptyd hamujący Bax, V5

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection.

• Synonim(y): BIP-V5, H-VPMLK-OH
• Wzór empiryczny (zapis Hilla): C₂₇H₅₀N₆O₆S
• Masa cząsteczkowa: 586.79
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• Poziom jakości: 100
• Próba: ≥95% (HPLC)
• Formularz: lyophilized solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; desiccated (hygroscopic); protect from light
• kolor: white
• rozpuszczalność: water: 1 mg/mL
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection. Functions as effectively as the Caspase Inhibitor VI (Z-VAD-FMK; Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also effectively blocks caspase-independent necrotic cell death. Shown to be Ku70 competitive, interact with Bax, prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days). Negative control peptide is also available (Cat. No. 196811).

A cell-permeable pentapeptide derived from the Ku70-Bax inhibiting domain that has been shown to offer protection against apoptosis in cells of human, mouse, rant, and porcine origin. Reported to function as effectively as the caspase inhibitor, Z-VAD-FMK (Caspase Inhibitor VI, Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also reported to effectively block caspase-independent necrotic cell death. Shown to interact with Bax in a Ku70-competitive manner and prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days).

Działania biochem./fizjol.

Cell permeable: yes

Primary Target
Bax mediated apoptosis

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50-200 µM against Bax mediated apoptosis

Opakowanie

Packaged under inert gas

Ostrzeżenie

Toxicity: Standard Handling (A)

Sekwencja

H-Val-Pro-Met-Leu-Lys-OH

Rekonstytucja

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Storage in glass vials is not recommended as loss of activity may occur.

Inne uwagi

Yoshida, T., et al. 2004. Biochem. Biophys. Res. Commun.321, 961.
Sawada, M., et al. 2003. Nature Cell Biol.5, 352.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable