17-10263
ChIPAb+ JMJD6 - ChIP Validated Antibody and Primer Set
from rabbit, purified by affinity chromatography
Synonim(y):
JMJD, jumonji domain containing 6, Phosphatidylserine receptor, Lysyl-hydroxylase JMJD6, Jumonji domain-containing protein 6, JmjC domain-containing protein 6, Peptide-lysine 5-dioxygenase JMJD6, Histone arginine demethylase JMJD63, Protein PTDSR
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.32
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
human
reaktywność gatunkowa (przewidywana na podstawie homologii)
Xenopus (based on 100% sequence homology)
producent / nazwa handlowa
ChIPAb+
Upstate®
metody
ChIP: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
Opis ogólny
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+JMJD1C set includes the JMJD1C antibody, a Normal Rabbit IgG, and control primers which amplify a 110 bp region of ChIP Primers, human β-globin. The JMJD1C and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of JMJD1C -associated chromatin.
The ChIPAb+JMJD1C set includes the JMJD1C antibody, a Normal Rabbit IgG, and control primers which amplify a 110 bp region of ChIP Primers, human β-globin. The JMJD1C and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of JMJD1C -associated chromatin.
JmjC domain-containing protein 6 (JMJD6) is a dioxygenase belonging to group of proteins known as the Jmjc-domain containing proteins. This family of proteins is a group of redox enzymes specifically involved in catalyzing oxidation reactions, and are characterized by the presence of a highly conserved jumonji C (Jmjc)-domain. JMJD6 can function both as a lysyl-hydroxylase and a histone arginine demethylase. As a lysyl-hydroxylase, JMJD6 catalyzes 5-hydroxylation of target protein lysine residues. As an arginine demethylase, JMJD6 has a critical part to play in histone code by demethylating of histone H3 and histone H4. It has also been shown to mediate U2AF2/U2AF65 5-hydrozylation, thereby functioning as a regulator for RNA splicing through affecting U2AF2/U2AF65 pre-mRNA activity. JMJD6 is a critical component of embryogenesis for the differentiation of various organs, and may act as a regulator for hematopoietic regulation and macrophage cytokine response. High expression of JMJD6 has been observed in heart, kidney and skeletal muscle. Medium to low expression has been seen in brain, liver, lung, ovary, pancreas, placenta, prostate, testis, thymus, and spleen.
Specyficzność
This antibody recognizes JMJD6.
Immunogen
Linear peptide corresponding to human JMJD6.
Zastosowanie
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1.4 µg of either Normal Rabbit IgGor Anti-JMJD6 and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of JMJD6 associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin as a positive locus, and β-actin promoter primers as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa nuclear extract was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-JMJD6 (1 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system.
Arrow indicates JMJD6 (~46 kDa). (Figure 3).
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of A431, HeLa cells using Anti-JMJD6 (Red). Actin filaments have been labeled with Alexa Fluor 488 dye-
Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus. (Figure 4).
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1.4 µg of either Normal Rabbit IgGor Anti-JMJD6 and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of JMJD6 associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin as a positive locus, and β-actin promoter primers as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa nuclear extract was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-JMJD6 (1 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system.
Arrow indicates JMJD6 (~46 kDa). (Figure 3).
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of A431, HeLa cells using Anti-JMJD6 (Red). Actin filaments have been labeled with Alexa Fluor 488 dye-
Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus. (Figure 4).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Histone Modifying Proteins
This ChIPAb+ JMJD6 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Opakowanie
25 assays per set. Recommended use: 1.4 µg (2 µL) of antibody per chromatin immuno-precipitation (dependent upon biological context).
Jakość
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1.4 µg of either Normal Rabbit IgG or Anti-JMJD6 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of JMJD6 associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1.4 µg of either Normal Rabbit IgG or Anti-JMJD6 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of JMJD6 associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Opis wartości docelowych
~46 kDa observed. Uncharacterized crossreacting band observed below the 46 kDa target band in some lysates.
Postać fizyczna
Affinity purified
Anti-JMJD6 (rabbit polyclonal). One vial containing 50 µL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C.
Concentration: 0.7 mg/mL
Normal Rabbit IgG. One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, human β-globin. One vial containing 75 μL of each primer (5 μM) specific for the human β-globin promoter. Store at -20°C.
FOR: AGG ACA GGT ACG GCT GTC ATC
REV: TTT ATG CCC AGC CCT GGC TC
Concentration: 0.7 mg/mL
Normal Rabbit IgG. One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, human β-globin. One vial containing 75 μL of each primer (5 μM) specific for the human β-globin promoter. Store at -20°C.
FOR: AGG ACA GGT ACG GCT GTC ATC
REV: TTT ATG CCC AGC CCT GGC TC
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Komentarz do analizy
Control
Includes normal rabbit IgG and primers specific for human β-globin.
Includes normal rabbit IgG and primers specific for human β-globin.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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