1.52006

Chromolith^® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 2 mm HPLC Column

• Synonim(y): Chromolith^® Performance RP-18 endcapped 100-2 HPLC column
• Kod UNSPSC: 41115709
• NACRES: SB.52

Właściwości

• product name: Chromolith^® RP-18 endcapped, L × I.D. 100 mm x 2 mm HPLC column
• Materiały: PEEK column
• Poziom jakości: 100
• agency: suitable for USP L1
• linia produktu: Chromolith^®
• Właściwości: endcapped
• opakowanie: 1 ea of
• zakres etykietowania: 18% Carbon loading
• Parametry: 200 bar pressure; 45 °C max. temp.
• metody: HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• kolumna dł. × śr. wewn.: 10 cm × 2 mm
• macierz: monolithic silica
• grupa aktywna macierzy: C18 bonding phase
• wielkość porów: 1.5 μm macroporosity; 13 nm mesoporosity
• operating pH range: 2-7.5
• metoda separacji: reversed phase
• temp. przechowywania: no temp limit

Opis

Opis ogólny

Chromolith^® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^® Performance RP-18 endcapped 100-2 columns have macropores with 1.5 μm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith^® Performance RP-18 endcapped 100-2 columns fall under the USP L1 Classification. The column efficiency is comparable to a 3-4 μm particle size fully porous particulate column. Chromolith^® Performance RP-18 endcapped 100-2 columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 2 mm I.D. is the best choice for fast separations of complex samples on any U/HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^® RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52009] with the Chromolith^® Guard Cartridge Holder (kit) 5 mm x 2 mm [1.52004]
The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Zastosowanie

• Rapid Determination of 30 Polyphenols in Tongmai Formula: This study utilized the Chromolith^® RP-18 endcapped column in a liquid chromatography-tandem mass spectrometry setup to analyze 30 polyphenolic compounds in a traditional Chinese medicine formula, demonstrating its application in pharmaceutical analysis and quality control of complex herbal formulations (Wang et al., 2017).


• Ultra-fast gradient LC method for omeprazole analysis using a monolithic column: The Chromolith^® RP-18 endcapped column was employed in an ultra-fast gradient liquid chromatography method for the analysis of omeprazole in enteric-coated pellets, highlighting its efficiency in pharmaceutical quality control and rapid method development (Borges et al., 2010).

Komentarz do analizy

Appearance: passes test
Pressure: ≤ 55 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 100000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.90 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.30 - 2.60

Informacje prawne

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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