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113200

Sigma-Aldrich

Mysz anty-α-aktyna mAb (1A4)

liquid, clone 1A4, Calbiochem®

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

100

rodzaj przeciwciała

primary antibodies

klon

1A4, monoclonal

Formularz

liquid

zawiera

≤0.1% sodium azide as preservative

reaktywność gatunkowa

baboon, rabbit, human

producent / nazwa handlowa

Calbiochem®

warunki przechowywania

do not freeze

izotyp

IgG2a

Warunki transportu

wet ice

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... ACTA1(58)

Opis ogólny

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
  • Antibody Target Gene Symbol: ACTA2
  • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
  • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
  • Hu Entrez ID: 59 (Related Antibodies: 113200)
  • Rat Entrez ID: 81633
    • This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

    Immunogen

    Human Smooth Muscle
    a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

    Zastosowanie

    Frozen Sections (1:50-1:75)

    Immunoblotting (see comments)

    Paraffin Sections (1:50-1:75)

    Opakowanie

    Please refer to vial label for lot-specific concentration.

    Ostrzeżenie

    Toxicity: Standard Handling (A)

    Postać fizyczna

    In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

    Komentarz do analizy

    Negative Control
    Fibroblasts, striated muscle, or myocardium
    Positive Control
    Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

    Inne uwagi

    Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
    Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

    Informacje prawne

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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    Kod klasy składowania

    12 - Non Combustible Liquids

    Klasa zagrożenia wodnego (WGK)

    WGK 2

    Temperatura zapłonu (°F)

    Not applicable

    Temperatura zapłonu (°C)

    Not applicable

    Certyfikaty analizy (CoA)

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