CP01
Anti-Actin (Ab-1) Mouse mAb (JLA20)
liquid, clone JLA20, Calbiochem®
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.43
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
JLA20, monoclonal
Postać
liquid
zawiera
≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL)
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
izotyp
IgM
Warunki transportu
wet ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... ACTB(60)
Opis ogólny
Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1 Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN Entrez Gene Name: actin, alpha 1, skeletal muscle Hu Entrez ID: 58, 71 (Related Antibodies: CP64) Mu Entrez ID: 11459 ;-; 11465 Rat Entrez ID: 29437 ;-; 287876
Immunogen
Chicken
chicken cytoskeletal proteins
Zastosowanie
Immunoblotting (1:5000-1:20000)
Immunofluorescence (1:10000, see application references)
Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Immunofluorescence (1:10000, see application references)
Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Ostrzeżenie
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Postać fizyczna
100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
Rekonstytucja
Following initial thaw, aliquot ascites and freeze (-20°C).
Komentarz do analizy
Positive Control
All animal cell lines
All animal cell lines
Inne uwagi
Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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