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09-864

Sigma-Aldrich

Anti-JHDM1B Antibody

from rabbit, purified by affinity chromatography

Synonim(y):

JMJD, lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B, JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein, F-box and leucine-rich repeat protein 10, protein containing CXXC domain 2, ju

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

oczyszczone przez

affinity chromatography

reaktywność gatunkowa

mouse, human, rat

reaktywność gatunkowa (przewidywana na podstawie homologii)

chimpanzee (based on 100% sequence homology), rabbit (based on 100% sequence homology), porcine (based on 100% sequence homology), horse (based on 100% sequence homology)

metody

immunocytochemistry: suitable
western blot: suitable

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... KDM2B(84678)

Opis ogólny

JmjC domain-containing histone demethylation protein 1B, also known as Lysine-specific demethylase 2B, is a nuclear enzyme encoded by the JHDM1B gene (also known as KDM2B) in human. JHDM1A/KDM2B exhibits H3K36-specific demethylase activity with a preference for H3K36me2 over H3K36me and H3K36me3 (PMID 16362057). JHDM1A/KDM2B is also reported to negatively regulate cell proliferation by targeting rDNA and thereby repressing the transcription of rRNA in a JmjC-domain-dependent manner (PMID 17994099).

Immunogen

KLH-conjugated linear peptide corresponding to human JHDM1B.

Zastosowanie

Anti-JHDM1B Antibody is a rabbit polyclonal antibody for detection of JHDM1B also known as lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B & has been validated in WB, ICC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected JHDM1B in NIH/3T3 and HeLa cells.
Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected JHDM1B in A431 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors

Jakość

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected JHDM1B on 10 µg of HeLa nuclear extract.

Opis wartości docelowych

~165 kDa observed. An uncharacterized lower molecular weight band may be observed in some lysates.

Postać fizyczna

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HeLa nuclear extract

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Tsuyoshi Fukuda et al.
Molecular and cellular neurosciences, 46(3), 614-624 (2011-01-12)
Histone methylation is the important transcription regulatory system that affects mammalian development and cell differentiation. Alterations in epigenetic gene regulation are associated with disease. Fbxl10 (F-box and leucine-rich repeat protein 10) is a JmjC domain-containing histone demethylase. Although Fbxl10 has
IPO8 and FBXL10: new reference genes for gene expression studies in human adipose tissue.
Hurtado del Pozo, C; Calvo, RM; Vesperinas-Garcia, G; Gomez-Ambrosi, J; Fruhbeck et al.
Obesity (Silver Spring, Md.) null
Manabu Ozawa et al.
Biology of reproduction, 94(4), 92-92 (2016-03-18)
The F-box and leucine-rich repeat protein 10 (Fbxl10) gene encodes a protein that catalyzes demethylation of H3K4 and H3K36. In this study, we show the important roles of FBXL10 as a histone demethylase in sustainable sperm production using mice in
Yangyang Yang et al.
Cell death discovery, 7(1), 328-328 (2021-11-01)
F-box and leucine-rich repeat protein 10 (FBXL10) has been reported to play a regulatory role in the initiation and development of breast cancer. Bioinformatics analyses revealed that FBXL10 may involve in the process of cytoskeleton organization. This research aimed to
Nefeli Zacharopoulou et al.
Cancer biology & therapy, 21(6), 533-540 (2020-03-17)
Recent studies revealed that the histone demethylase KDM2B regulates the epithelial markers E-Cadherin and ZO-1, the RhoA/B/C-small-GTPases and actin cytoskeleton organization, in DU-145 prostate- and HCT-116 colon-tumor cells. Here we addressed the role of KDM2B in the activation of Focal

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