07-478
Anti-SNF2β/BRG1 Antibody
serum, Upstate®
Synonim(y):
Probable global transcription activator SNF2L4, ATP-dependent helicase SMARCA4, SNF2-beta, BRG-1, Mitotic growth and transcription activator, Brahma protein homolog 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A m
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
rat, mouse, human
opakowanie
antibody small pack of 25 μL
producent / nazwa handlowa
Upstate®
metody
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
ambient
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... SMARCA4(6597)
Powiązane kategorie
Opis ogólny
The SNF2 family of proteins are ATP dependant chromatin remodeling enzymes. The gene encoding this protein is also refered to as SMARCA4.
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
Specyficzność
This antibody recognizes the Near N-Terminus of SNF2B
Immunogen
GST-tagged recombinant protein corresponding to the Near N-Terminus of human SNF2β.
Zastosowanie
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected BRG1 in HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Transcription Factors
Cell Cycle, DNA Replication & Repair
Transcription Factors
Use Anti-SNF2β/BRG1 Antibody (rabbit polyclonal antibody) validated in IP, WB to detect SNF2β/BRG1 also known as Probable global transcription activator SNF2L4, ATP-dependent helicase SMARCA4, SNF2-beta, BRG-1.
Jakość
Evaluated by Western Blotting in HELA NE cell lysate.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected SNF2B/BRG1 in 10 µg of HELA NE cell lysate.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected SNF2B/BRG1 in 10 µg of HELA NE cell lysate.
Opis wartości docelowych
200 kDa
Postać fizyczna
Rabbit polyclonal in buffer containing with 0.05% sodium azide with 30% glycerol.
Unpurified
Przechowywanie i stabilność
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Komentarz do analizy
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Activation of ATP-binding cassette transporter A1 transcription by chromatin remodeling complex.
Huuskonen, J; Vishnu, M; Fielding, PE; Fielding, CJ
Arteriosclerosis, Thrombosis, and Vascular Biology null
Cutting edge: SWISNF mediates antisense Igh transcription and locus-wide accessibility in B cell precursors.
Osipovich OA, Subrahmanyam R, Pierce S, Sen R, Oltz EM
Journal of immunology (Baltimore, Md. : 1950) (1950)
Joel Vincent Fair et al.
BMC developmental biology, 16(1), 27-27 (2016-08-04)
The Hedgehog (HH) signalling pathway regulates cardiomyogenesis in vivo and in differentiating P19 embryonal carcinoma (EC) cells, a mouse embryonic stem (mES) cell model. To further assess the transcriptional role of HH signalling during cardiomyogenesis in stem cells, we studied
Germline and somatic SMARCA4 mutations characterize small cell carcinoma of the ovary, hypercalcemic type.
Witkowski, Leora, et al.
Nature Genetics, 46, 438-443 (2014)
Petar Jelinic et al.
Nature genetics, 46(5), 424-426 (2014-03-25)
Small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) is a rare, highly aggressive form of ovarian cancer primarily diagnosed in young women. We identified inactivating biallelic SMARCA4 mutations in 100% of the 12 SCCOHT tumors examined. Protein studies confirmed
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