07-1820
Anti-EEA1 Antibody
from rabbit, purified by affinity chromatography
Synonim(y):
early endosome antigen 1, early endosome-associated protein, early endosome antigen 1, 162kD, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
mouse, human
opakowanie
antibody small pack of 25 μL
metody
immunocytochemistry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
ambient
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... EEA1(8411)
Powiązane kategorie
Opis ogólny
Early endosome antigen 1 (EEA1) is a 170 kDa coiled-coil protein belonging to a group of early endosomal Rab5 effector proteins. EEA1 has a putative role in the tethering/docking of endocytic vesicles prior to their fusion with early endosomes. This protein is characterized as containing a zinc finger-like domain and coiled-coil regions. The zinc finger-like domain, also known as the FYVE finger, is a motif rich in cystine, is a necessary component of EEA1’s ability for endosomal targeting, and is specifically known for its dual interaction with Rab5 GTPase and phosphotidylinositol 3-phosphate (Ptdlns-3-P).
Specyficzność
Equine and Canine (99% sequence homology). Rat (98% sequence homology). Bovine (97% sequence homology).
This antibody recognizes EEA1.
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of human EEA1.
Zastosowanie
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
This Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.
Western Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells.
Jakość
Evaluated by Western Blot in BALB 3T3/A31 cell lysate.
Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate.
Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate.
Opis wartości docelowych
~ 170 kDa
Postać fizyczna
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Komentarz do analizy
Control
BALB 3T3/A31 cell lysate
BALB 3T3/A31 cell lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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