07-169-I

Anti-iPLA2 Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): iPLA2, 85/88 kDa calcium-independent phospholipase A2 (EC:3.1.1.4), CaI-PLA2, Group VI phospholipase A2, GVI PLA2, Intracellular membrane-associated calcium-independent phospholipase A2 beta, iPLA2-beta, Patatin-like phospholipase domain-containing prote
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): hamster (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• metody: immunohistochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NP_001186491
• numer dostępu UniProt: O60733
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... PLA2G6(8398)

Opis

Opis ogólny

Phospholipase A2 (PLA2) enzymes constitute the largest class of phospholipases, members of which catalyze the hydrolysis of the phospholipid sn-2 ester bond, generating a free fatty acid and a lysophospholipid. PLA2 enzymes are divided into 14 groups (I-XIV), including 20 subgroups (e.g. IA, IB, VIA-1, VIA-2, VIB, etc.), based on sequence homology. Historically, PLA2 enzymes have also been classified based on their biological characteristics into three types, secretory (sPLA2), cytosolic Ca2+-dependent (cPLA2), and cytosolic Ca2+-independent (iPLA2), when sequence data were not available. (PMID 12401193 & 12694560). Cat. No. 07-169-I, Anti-iPLA2, is a purified rabbit polyclonal raised against immunogen sequence present in the group VI SH-iPLA2 & LH-iPLA2 (85/88 kDa calcium-independent phospholipase A2) isoforms, but not found in the 53.2 kDa Ankyrin-iPLA2-1 and 46.5 kDa Ankyrin-iPLA2-2 splice variants encoded by the same PLA2G6 (PLPLA9) gene (UniProt O60733). Immunogen sequence is 100% conserved in murine (NP_058611.1) and rodent (AAC53136.1) species.

Immunogen

Epitope: near C-terminal

KLH-conjugated linear peptide corresponding to human iPLA2 near the C-terminal.

Zastosowanie

Anti-iPLA2 Antibody is an antibody against iPLA2 for use inWestern Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected iPLA2 in human tonsil tissue.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Jakość

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected iPLA2 in 10 µg of human testis tissue lysate.

Opis wartości docelowych

~70 kDa observed (Ramanadham, S., et al. (2003). Biochemistry. 42(47):13929-13940). This antibody is expected to cross-react to isoforms LH-iPLA2 and SH-iPLA2 only.

Postać fizyczna

Affinity

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable