07-1231
Anti-Notch1 Antibody, Intracellular
serum, from rabbit
Synonim(y):
Neurogenic locus notch homolog protein 1 precursor, Notch (Drosophila) homolog 1 (translocation-associated), Notch 1, Notch homolog 1, translocation-associated (Drosophila), Translocation-associated notch protein TAN-1, Neurogenic locus notch homolog pro
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
serum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
human
spodziewany brak reakcji z
mouse
metody
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... NOTCH1(4851)
Opis ogólny
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
Specyficzność
Notch 1, intracellular. No reactivity against human Notch 2.
Reactivity with other species has not been determined.
Immunogen
Synthetic peptide from human Notch 1.
Zastosowanie
ELISA: 1:20,000-1:100,000 dilution from a previous lot was used in a standard sandwich ELISA assay against the peptide immunogen.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining IGF-1 With Epitope Retrieval: Lipoma.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining IGF-1 With Epitope Retrieval: Lipoma.
Use Anti-Notch1 Antibody, Intracellular (Rabbit Polyclonal Antibody) validated in ELISA, WB, IHC(P) to detect Notch1 also known as Neurogenic locus notch homolog protein 1 precursor.
Jakość
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected NOTCH1, intracellular (Cleaved form) on 10 μg of HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected NOTCH1, intracellular (Cleaved form) on 10 μg of HeLa lysates.
Opis wartości docelowych
~80 kDa
Powiązanie
Replaces: 04-1046
Postać fizyczna
Rabbit polyclonal IgG serum in buffer containing in 0.02 M Potassium phosphate, 0.15 M NaCl with 0.01% sodium azide.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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The participation of NOTCH signaling in invasive cervical cancer (ICC) remains controversial since both tumor suppressive and oncogenic properties have been described. Additionally, the role of NUMB, a negative regulator of NOTCH, remains unclear in ICC. We aimed to investigate
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Gland surgery, 10(11), 3116-3127 (2021-12-21)
Deltex 1 (DTX1) is a single transmembrane protein with ubiquitin E3 ligase activity which has been found to play a role in the development of several cancers. We aimed to investigate the associations between DTX1 and breast cancer (BC). We
Sunkuan Hu et al.
Oncology letters, 15(5), 6171-6178 (2018-04-05)
Due to the complex function of the Notch signal pathway in gastric cancer (GC), the association between Notch homolog 1 (Notch1) intracellular domain (NICD) and differentiation of GC remains unknown. The present study aimed to investigate the potential association between
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