06-711
Anti-FADD Antibody
Upstate®, from rabbit
Synonim(y):
FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
human
producent / nazwa handlowa
Upstate®
metody
immunohistochemistry: suitable (paraffin)
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... FADD(8772)
Opis ogólny
FAS-associated death domain protein (UniProt: Q13158; also known as FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD) is encoded by the FADD (also known as MORT1, GIG3) gene (Gene ID: 8772) in human. FADD is an apoptotic adaptor molecule that bridges death receptor signaling to the caspase cascade. It is expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. FADD contains a death domain at its C-terminus and a death effector domain (DED) at its N-terminal. The death domain is responsible for FADD binding to the activated trimerized Fas receptor. FADD recruits procaspase-8 through its N-terminal DED, which is then activated following the cleavage of pro domain. The death inducing signaling complex (DISC) formed by Fas receptor, FADD, and caspase-8 is essential in triggering apoptotic events in cells. Human FADD is phosphorylated mainly at Ser194. Defects in FADD gene have been linked to recurrent infections, hepatic dysfunction, and cardiovascular malformations. These subjects display high circulating levels of CD4(-) and CD8(-) cells and elevated levels of IL-10.
Specyficzność
This polyclonal antibody detects FADD protein in human cells.
Immunogen
His-tagged full-length human recombinant FAS-associated death domain protein.
Zastosowanie
Detect FAS-associated death domain protein using this rabbit polyclonal Anti-FADD, Cat. No. 06-711. It has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected FADD in human pancreas, human kidney, and human prostate tissue.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Jakość
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected FADD in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected FADD in 10 µg of Jurkat cell lysate.
Opis wartości docelowych
~26 kDa observed; 23.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Powiązanie
Replaces: 04-1113
Postać fizyczna
ImmunoAffinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Komentarz do analizy
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Inne uwagi
Concentration: Please refer to lot specific datasheet.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Molecular determinants of kinase pathway activation by Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand.
Varfolomeev, E; Maecker, H; Sharp, D; Lawrence, D; Renz, M; Vucic, D; Ashkenazi, A
The Journal of Biological Chemistry null
Xin Xu et al.
Cell death & disease, 11(2), 94-94 (2020-02-07)
Adult T-cell leukemia/lymphoma (ATLL) is a malignancy of mature T cells associated with chronic infection by human T-cell lymphotropic virus type-1 (HTLV-1). ATLL patients with aggressive subtypes have dismal outcomes. We demonstrate that ATLL cells co-opt an early checkpoint within
Teruki Yanagi et al.
PloS one, 10(3), e0119404-e0119404 (2015-03-20)
While PCTAIRE1/PCTK1/Cdk16 is overexpressed in malignant cells and is crucial in tumorigenesis, its function in apoptosis remains unclear. Here we investigated the role of PCTAIRE1 in apoptosis, especially in the extrinsic cell death pathway. Gene-knockdown of PCTAIRE1 sensitized prostate cancer
Peifeng Li et al.
The Journal of biological chemistry, 285(29), 22713-22722 (2010-05-21)
MADD plays an essential role in cancer cell survival. Abrogation of endogenous MADD expression results in significant spontaneous apoptosis and enhanced susceptibility to tumor necrosis factor alpha-related apoptosis-inducing ligand (TRAIL)-induced apoptosis. However, the regulation of MADD function is largely unknown.
Mélanie Simoes Eugénio et al.
Frontiers in molecular biosciences, 8, 645134-645134 (2021-05-04)
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a well-known apoptosis inducer and a potential anticancer agent. When caspases and inhibitors of apoptosis proteins (IAPs) are inhibited, TRAIL induces necroptosis. Molecular mechanisms of necroptosis rely on kinase activation, and on the
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