06-543
Anti-FAK Antibody
Upstate®, from rabbit
Synonim(y):
FADK 1, PTK2 protein tyrosine kinase 2, Protein-tyrosine kinase 2, focal adhesion kinase 1
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
human, hamster, mouse, rat
spodziewany brak reakcji z
avian
producent / nazwa handlowa
Upstate®
metody
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... PTK2(5747)
Opis ogólny
Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and it′s phosphorylation states have been implicated in cancer metastasis and tumor cell survival and adhesion-independent growth. Additionally, recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
Immunogen
pGEX-derived fusion protein containing residues 748-1053 of human FAK
Zastosowanie
Immunocytochemistry: 10 μg/mL of a previous lot showed positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde.
Immunoprecipitation: 4 μg of a previous lot immunoprecipitated FAK from 500 μg of murine 3T3/A31 RIPA lysate.
Immunoprecipitation: 4 μg of a previous lot immunoprecipitated FAK from 500 μg of murine 3T3/A31 RIPA lysate.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
This Anti-FAK Antibody is validated for use in WB, IP, IC for the detection of FAK.
Jakość
Evaluated by western blot on RIPA lysates from murine 3T3/A31 or human A431 RIPA cell lysate.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FAK in RIPA lysates from murine 3T3/A31 and previously in human A431 RIPA cell lysate.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FAK in RIPA lysates from murine 3T3/A31 and previously in human A431 RIPA cell lysate.
Opis wartości docelowych
125 kDa
Powiązanie
Replaces: 04-591
Postać fizyczna
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). Store at -20°C.
Przechowywanie i stabilność
Stable for 1 year at from date of receipt.
Komentarz do analizy
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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