05-939
Anti-LSD1/BHC110 Antibody
ascites fluid, Upstate®
Synonim(y):
BRAF35-HDAC complex protein BHC110, FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit, Flavin-containing amine oxidase domain-containing protein 2, amine oxidase (flavin containing) domain 2, lysine (K)-specific demethylase 1, lysine-specific hist
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
monoclonal
reaktywność gatunkowa
mouse, human
producent / nazwa handlowa
Upstate®
metody
ChIP: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... KDM1A(23028)
Opis ogólny
Histone demethylase that specifically demethylates ′Lys-4′ of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted ′Lys-4′ of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 ′Lys-4′ on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate ′Lys-9′ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Specyficzność
Recognizes LSD1, Mr 110 kDa.
Immunogen
GST fusion protein corresponding to residues 23-138 of human LSD1.
Zastosowanie
Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB.
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.
Immunoprecipitation:
Recommended.
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)
Immunofluorescence:
Recommended.
This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.
Immunoprecipitation:
Recommended.
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)
Immunofluorescence:
Recommended.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Chromatin Biology
Histones
Chromatin Biology
Jakość
Routinely evaluated by western blot.
Western Blot Analysis:
1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.
Western Blot Analysis:
1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.
Opis wartości docelowych
110 kDa
Powiązanie
Replaces: CBL770
Postać fizyczna
Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.
Unpurified
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Komentarz do analizy
Control
HeLa cell lysate.
HeLa cell lysate.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Mohamed-Ali Hakimi et al.
The Journal of biological chemistry, 278(9), 7234-7239 (2002-12-21)
Eukaryotic genes are under the control of regulatory complexes acting through chromatin structure to control gene expression. Here we report the identification of a family of multiprotein corepressor complexes that function through modifying chromatin structure to keep genes silent. The
Lysine-specific histone demethylase 1 inhibitors control breast cancer proliferation in ER?-dependent and -independent manners.
Pollock, JA; Larrea, MD; Jasper, JS; McDonnell, DP; McCafferty, DG
ACS chemical biology null
Eric Metzger et al.
Nature, 437(7057), 436-439 (2005-08-05)
Gene regulation in eukaryotes requires the coordinate interaction of chromatin-modulating proteins with specific transcription factors such as the androgen receptor. Gene activation and repression is specifically regulated by histone methylation status at distinct lysine residues. Here we show that lysine-specific
Xing Zeng et al.
Genes & development, 30(16), 1822-1836 (2016-08-28)
Brown adipocytes display phenotypic plasticity, as they can switch between the active states of fatty acid oxidation and energy dissipation versus a more dormant state. Cold exposure or β-adrenergic stimulation favors the active thermogenic state, whereas sympathetic denervation or glucocorticoid
T Boulding et al.
Scientific reports, 8(1), 73-73 (2018-01-10)
Complex regulatory networks control epithelial-to-mesenchymal transition (EMT) but the underlying epigenetic control is poorly understood. Lysine-specific demethylase 1 (LSD1) is a key histone demethylase that alters the epigenetic landscape. Here we explored the role of LSD1 in global epigenetic regulation
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