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05-584

Sigma-Aldrich

Anti-Tie2/TEK Antibody, clone Ab33

clone Ab33, Upstate®, from mouse

Synonim(y):

CD202b antigen, TEK tyrosine kinase, endothelial, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, venous malformations, multip

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

Ab33, monoclonal

reaktywność gatunkowa

human, rat, pig, mouse

producent / nazwa handlowa

Upstate®

metody

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG1κ

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... TEK(7010)

Opis ogólny

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Specyficzność

Recognizes Tie2/TEK, Mr 145kDa

Immunogen

Fusion protein corresponding to residues 1-745 of human Tie2/TEK with a C-terminal His6-tag purified from infected SF-9 cells. Clone Ab33

Zastosowanie

Immunoprecipitation: This antibody immunoprecipitated Tie2/TEK, as reported by an independent laboratory.

Immunohistochemistry: Reported to detect Tie2/TEK in frozen, Triton-treated sections.
This antibody is not suitable for paraffin-embedded sections.
This Anti-Tie2/TEK Antibody, clone Ab33 is validated for use in WB, IP, IH for the detection of Tie2/TEK.

Jakość

Evaluated by western blot on RIPA lysates from HUVEC cells.

Western Blot Analysis: 0.2-1 μg/mL of this antibody detected Tie2/TEK in RIPA lysates from HUVEC cells.

Opis wartości docelowych

145 kDa

Postać fizyczna

Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Mary Anna Venneri et al.
Blood, 109(12), 5276-5285 (2007-03-01)
Tumor-infiltrating myeloid cells, including tumor-associated macrophages (TAMs), have been implicated in tumor progression. We recently described a lineage of mouse monocytes characterized by expression of the Tie2 angiopoietin receptor and required for the vascularization and growth of several tumor models.
Daisuke Sakai et al.
JOR spine, 1(2), e1018-e1018 (2018-06-27)
Recently, Tie2/TEK receptor tyrosine kinase (Tie2 or syn. angiopoietin-1 receptor) positive nucleus pulposus progenitor cells were detected in human, cattle, and mouse. These cells show remarkable multilineage differentiation capacity and direct correlation with intervertebral disc (IVD) degeneration and are therefore
Haixia Gong et al.
Scientific reports, 7, 42127-42127 (2017-02-16)
Human endothelial cells (ECs) are widely used to study mechanisms of angiogenesis, inflammation, and endothelial permeability. Targeted gene disruption induced by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-Associated Protein 9 (Cas9) nuclease gene editing is potentially an important tool for
Hiroko Fujii et al.
Arteriosclerosis, thrombosis, and vascular biology, 26(11), 2476-2482 (2006-08-26)
C-reactive protein (CRP) has been suggested to participate in the development of atherosclerosis, in part, by promoting endothelial dysfunction and impairing endothelial progenitor cell (EPC) survival and differentiation. In the present study, we evaluated the effects of CRP on antioxidative
Delivery of therapeutic siRNA to the lung endothelium via novel Lipoplex formulation DACC.
Fehring, V; Schaeper, U; Ahrens, K; Santel, A; Keil, O; Eisermann, M; Giese, K; Kaufmann, J
Molecular Therapy null

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