04-1557

Anti-SIRT1 Antibody, clone 10E4

clone 10-E-4, from mouse

• Synonim(y): SIR2-like protein 1, sir2-like 1, sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, sirtuin 1, sirtuin type 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klon: 10-E-4, monoclonal
• application: ChIP; ICC; IHC; IP; WB
• reaktywność gatunkowa: mouse, human
• metody: ChIP: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 15

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 10-E-4, monoclonal
• reaktywność gatunkowa: mouse, human
• opakowanie: antibody small pack of 25 μL
• metody: ChIP: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• numer dostępu NCBI: NP_036370.2
• numer dostępu UniProt: Q96EB6
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SIRT1(23411)

Opis

Opis ogólny

Sir2 is short for Silent mating type Information Regulation-2. Sir2 (whose homolog in mammals is known as SIRT1, SIR2L1 or Sir2α) is the namesake of a family of closely related enzymes, the sirtuins. Members of this family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism′s response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". In mammals, SIRT1 (the mammalian homolog of Sir2) has been shown to deacetylate and thereby deactivate the p53 protein.

Specyficzność

This antibody recognizes SIRT1.

Immunogen

Epitope: Unknown

His-tagged recombinant protein corresponding to human SIRT1.

Zastosowanie

Does not react in mouse in IHC. Mouse sequence is 100% identical to immunogen, so it is predicted to work in western blotting.

Immunoprecipitation: Reported by independent researcher using representative lot.
Chromatin Immunopreiciptiation: Reported by independent researcher using representative lot.
Immunohistochemistry: Reported by independent researcher using representative lot.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-SIRT1 Antibody, clone 10E4 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC, ChIP to detect SIRT1 also known as sirtuin (silent mating type information regulation 2 homolog) 1, sirtuin 1.

Jakość

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected SIRT1 on 10 µg of HeLa cell lysate.

Opis wartości docelowych

~ 110 kDa

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HeLa cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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