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Merck
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Key Documents

TOX4

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Neutral Red based

동의어(들):

NR uptake assay, Neutral red assay

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.84

형태

liquid

사용

 kit sufficient for 1,000 tests

포장

pkg of 1 kit

저장 조건

dry at room temperature

λmax

540 nm

응용 분야

cell analysis
detection

검출 방법

colorimetric

저장 온도

2-8°C

일반 설명

For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.

애플리케이션

In Vitro Toxicology Assay Kit, Neutral Red based has been used:
  • to measure the lysosomal enzyme activity in HB8902 cells
  • to study the cell viability of human alveolar epithelial cells
  • to study the cell viability in fibroblasts

생화학적/생리학적 작용

Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.

픽토그램

FlameHealth hazardExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Carc. 1B - Flam. Liq. 3 - Skin Sens. 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point (°F)

73.4 °F

Flash Point (°C)

23 °C


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

D Triglia et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 27A(3 Pt 1), 239-244 (1991-03-01)
A new three-dimensional human skin model consisting of several layers of actively dividing and metabolically active human neonatal foreskin-derived fibroblasts and epidermal keratinocytes grown on nylon mesh has been used to assess the in vitro toxicity of test agents from
Guillermo Repetto et al.
Nature protocols, 3(7), 1125-1131 (2008-07-05)
The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of
Barbara Kazuń et al.
BMC veterinary research, 16(1), 216-216 (2020-06-27)
The aim of the study has been to compare the effect of dietary supplementation of β-1,3/1,6-glucan, Lactobacillus plantarum bacteria or their mixture on the growth performance, selected parameters of the immune system as well as the liver and intestinal histology
Alessandro Di Cerbo et al.
Antibiotics (Basel, Switzerland), 10(8) (2021-08-28)
One of the most-used food contact materials is stainless steel (AISI 304L or AISI 316L), owing to its high mechanical strength, cleanability, and corrosion resistance. However, due to the presence of minimal crevices, stainless-steel is subject to microbial contamination with
R Fautz et al.
Mutation research, 253(2), 173-179 (1991-10-01)
The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers

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