추천 제품
사용
1 kit sufficient for 1,000 tests
포장
pkg of 1 kit
저장 조건
dry at room temperature
λmax
570 nm
응용 분야
cell analysis
detection
검출 방법
colorimetric
저장 온도
2-8°C
일반 설명
This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.
애플리케이션
In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.
생화학적/생리학적 작용
Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3
표적 기관
Central nervous system, Respiratory system
Storage Class Code
3 - Flammable liquids
Flash Point (°F)
53.6 °F
Flash Point (°C)
12 °C
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문서
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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