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Merck
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Key Documents

CGD1

Sigma-Aldrich

Cell Growth Determination Kit, MTT based

동의어(들):

Mitochondrial activity assay

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

포장

pkg of 1 kit

기술

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

응용 분야

cell analysis
detection

검출 방법

colorimetric

배송 상태

dry ice

저장 온도

−20°C

일반 설명

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

애플리케이션

Cell Growth Determination Kit, MTT based has been used:
  • to measure the cell viability of preosteoblastic cells
  • in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
  • to determine the cell viability of primary cervical epithelial cells

포장

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

관련 제품

제품 번호
설명
가격

픽토그램

FlameHealth hazardExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3

표적 기관

Central nervous system

Storage Class Code

3 - Flammable liquids

Flash Point (°F)

53.6 °F - closed cup

Flash Point (°C)

12 °C - closed cup


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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