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Merck
모든 사진(1)

주요 문서

SAE0196

Sigma-Aldrich

Achromopeptidase from bacteria

free of DNA contaminants, suitable for Microbiome research

동의어(들):

Bacterial Protease

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About This Item

UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

bacterial

Quality Level

형태

lyophilized powder

특이 활성도

≥1000 U/mg

특징

DNA free

기술

DNA extraction: suitable

적합성

suitable for cell lysis

응용 분야

cell analysis

배송 상태

ambient

저장 온도

−20°C

일반 설명

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases. This product is free of detectable microbial DNA and is suitable for microbiome research

애플리케이션

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme, as well as Gram-negative organisms. Achromopeptidase has been shown to contain two bacteriolytic proteases.

The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminants are essential. This product undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

생화학적/생리학적 작용

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. Optimum pH is 8.5-9.

특징 및 장점

Contains two bacteriolytic proteases for efficient lysis of Gram-positive bacteria and Gram-negative organisms

Free of detectable microbial DNA, ensuring the accuracy of metagenomics and other culture-independent sequencing methods

Undergoes strict quality control testing to ensure the absence of detectable levels of contaminating microbial DNA

Optimum pH of 8.5-9 allows for use in a wide range of microbiome studies

단위 정의

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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