A3422

Achromopeptidase from bacteria

partially purified powder, ≥20,000 units/mg solid

• 동의어(들): ACP, Achromopeptidase
• 효소 위원회 번호: 3.4.21.50 (BRENDA, IUBMB)
• MDL number: MFCD00130333
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 형태: partially purified powder
• Quality Level: 200
• 특이 활성도: ≥20,000 units/mg solid
• 분자량: 27 kDa
• 외래 활성: Collagenase, present
• 저장 온도: −20°C

설명

애플리케이션

Achromopeptidase from bacteria may be used:

• in the homogenization step for genomic DNA extraction from microbial biofilm
• in the lysis of gram-positive strains(
• for DNA extraction from Nocardia strains

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.

생화학적/생리학적 작용

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀ = 0.6 over 2 hours at 37 °C.

단위 정의

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

물리적 형태

Partially purified powder.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves