SAE0098

Lyticase from Arthrobacter luteus

free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder

• 동의어(들): (1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
• CAS Number: 37340-57-1
• MDL number: MFCD00131560
• UNSPSC 코드: 12352202
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Arthrobacter luteus)
• 형태: lyophilized powder
• 특이 활성도: ≥2000 units/mg protein
• 특징: DNA free
• 기술: cell based assay: suitable
• 적합성: suitable for cell lysis
• 응용 분야: diagnostic assay manufacturing
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.

Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

생화학적/생리학적 작용

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

단위 정의

One unit will produce a ΔA₈₀₀ of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable