SRE0007

Mutanolysin from Streptomyces globisporus ATCC 21553

Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret)

• 동의어(들): Mutanolysin
• CAS Number: 55466-22-3
• MDL number: MFCD00131635
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Streptomyces globisporus)
• Quality Level: 400
• 양식: lyophilized powder
• 특이 활성도: ≥4000 units/mg protein (biuret)
• 분자량: 23 kDa
• 농도: ≥40% protein
• 적합성: suitable for cell lysis
• 응용 분야: diagnostic assay manufacturing
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control

애플리케이션

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

생화학적/생리학적 작용

Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

단위 정의

One unit will produce a ΔA₆₀₀ of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

물리적 형태

Lyophilized powder containing Ficoll^® and sodium succinate buffer salts

법적 정보

Ficoll is a registered trademark of Cytiva

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1