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Merck
모든 사진(2)

Key Documents

SAB5801040

Sigma-Aldrich

Goat Anti-Mouse IgM(µ chain) Alpaca Heavy chain only Antibody

recombinant Alpaca(VHH) Nano antibody

동의어(들):

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

UNSPSC 코드:
12352203

생물학적 소스

alpaca

재조합

expressed in HEK 293 cells (Human cell line)

결합

unconjugated

항체 형태

affinity purified immunoglobulin

항체 생산 유형

secondary antibodies

클론

recombinant monoclonal

형태

lyophilized solid

분자량

100 kDa

종 반응성

Alpaca, mouse

기술

ELISA: 1:5000-1:20000
western blot: 1:5000-1:20000

크기

0.1 mL

색상

colorless

동형

VHH

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Goat Anti-Mouse IgM(µ chain) Alpaca Heavy chain only Antibody is designed to specifically detect mouse IgM. It is a monovalent, single domain, recombinant antibody fused to goat IgG Fc. Based on immunoelectrophoresis and/or ELISA, Goat Anti-Mouse IgM(µ chain) Alpaca Heavy chain only Antibody reacts with the µ chain of mouse IgM selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.


Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. IgM accounts for 5-10% of the immunoglobulin pool and is the predominant antibody in the primary immune response. Unlike IgG, IgM does not contain a hinge region but does contain an additional constant domain. The monomeric form IgM has a molecular weight of 180 KD. It is classically represented as a pentamer of the basic four chain structure held together by a J chain but can also exist in a hexameric form without the J chain and as a monomer on the surface of B-cells.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

특이성

Fc region of mouse IgM (µ chain specific)
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc

면역원

Recombinant Alpaca single variable domain secondary nano antibody

포장

100 μg in Glass bottle

물리적 형태

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

재구성

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

저장 및 안정성

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

기타 정보

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG

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픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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