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Merck
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Key Documents

18772

Sigma-Aldrich

Anti-Rabbit IgG - Atto 488 antibody produced in goat

whole molecule, 1 mg/mL protein

동의어(들):

Atto 488-[goat-Anti-rabbit IgG], whole molecule

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

결합

Atto 488 conjugate

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

포함

50% glycerol

종 반응성

rabbit

농도

1 mg/mL protein

기술

immunocytochemistry: 1:500
immunofluorescence: 1:200
immunohistochemistry: suitable

형광

λex 485 nm; λem 552 nm in PBS

배송 상태

wet ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

일반 설명

Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody antibody reats with rabbit IgG in vitro, in rabbit serum and biological fluids.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability. Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

면역원

Rabbit IgG

애플리케이션

Anti-Rabbit IgG has been used for immunocytochemistry applications at a dilution of 1:500 in neural precursor cells.
Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids via fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use rabbit IgG as the primary antibody. Atto 488 goat anti-rabbit IgG antibody was used for immunofluorescence to detect L. monocytogenes in mouse tissue sections following incubation with an anti-listeria antibody. Antibody was used at a 1:200 dilution.

물리적 형태

Atto 488 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

분석 메모

Unconjugated dye ≤5% of total fluorescence; dye-to-protein ratio >2.

법적 정보

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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