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Merck
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Key Documents

SAB4301134

Sigma-Aldrich

Anti-6-His antibody produced in rabbit

affinity isolated antibody

동의어(들):

6 His epitope tag, Anti His Antibody Sigma, Anti His Antibody Sigma - Anti-6×His antibody produced in rabbit

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

형태

buffered aqueous solution

분자량

46 kDa

농도

0.5 mg/mL

기술

western blot: 1:500-1:2000 (Cell Lysate)

동형

IgG

배송 상태

wet ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

일반 설명

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

특이성

The antibody detects transfected proteins contanining 6×His tag.

면역원

Synthetic peptide: 18XHis conjugated to KLH

애플리케이션

Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.
The Anti-6-His antibody has been used in western blotting.

생화학적/생리학적 작용

The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.

특징 및 장점

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

물리적 형태

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

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O-GlcNAcylation of fumarase maintains tumour growth under glucose deficiency
Wang T, et al.
Nature Cell Biology, 19(7), 833-833 (2017)
The FLAG? peptide, a versatile fusion tag for the purification of recombinant proteins.
Einhauer A and Jungbauer A
Journal of Biochemical and Biophysical Methods, 49(1-3), 455-465 (2001)
Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation
Jiang Y, et al.
Nature Cell Biology, 17(9), 1158-1158 (2015)
Ningning Shi et al.
Advanced biology, 6(11), e2200092-e2200092 (2022-07-13)
Site-specific incorporation of distinct noncanonical amino acids (ncAAs) into proteins via genetic code expansion in mammalian cells represents a new avenue for protein engineering. Reassigning three TAGs with the same ncAA in mammalian cells has previously been achieved using translational

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