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Merck
모든 사진(6)

Key Documents

SAB2702218

Sigma-Aldrich

Monoclonal Anti-6X His tag antibody produced in mouse

clone GT359, affinity isolated antibody

동의어(들):

Mouse Anti-Hexa Histidine tag

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.43

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

GT359, monoclonal

형태

buffered aqueous solution

종 반응성

human, mouse

농도

1mg/mL

기술

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: 500-3000

동형

IgG3

배송 상태

wet ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

일반 설명

6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein.

면역원

The immunogen used to generate this antibody corresponds to 6X His tag

애플리케이션

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Monoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting.

생화학적/생리학적 작용

6X His tag does not affect the structure and function of protein and can be used in antibody production. It is also used to purify proteins under native or denaturing form.

특징 및 장점

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

기타 정보

Purification: Affinity purified by Protein G

물리적 형태

Phosphate-buffered saline, no preservative added.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Disrupting CD147-RAP2 interaction abrogates erythrocyte invasion by Plasmodium falciparum.
Z Meng-Yao, et al.
Blood, 131(10), 1111-1121 (2018)
SKP2-and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity.
Yao F, et al.
Nature Communications, 9(1), 2269-2269 (2018)
Jordan Demone et al.
PloS one, 17(12), e0277668-e0277668 (2022-12-15)
Agroinfiltration is a method used in biopharming to support plant-based biosynthesis of therapeutic proteins such as antibodies and viral antigens involved in vaccines. Major advantages of generating proteins in plants is the low cost, massive scalability and the rapid yield
Molecular Biology of Streptococci (2007)
Meng-Yao Zhang et al.
Blood, 131(10), 1111-1121 (2018-01-21)
Effective vaccines against malaria caused by Plasmodium falciparum are still lacking, and the molecular mechanism of the host-parasite interaction is not fully understood. Here we demonstrate that the interaction of RAP2, a parasite-secreted rhoptry protein that functions in the parasitophorous

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