추천 제품
생물학적 소스
rat
Quality Level
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
3F10, monoclonal
양식
lyophilized
포장
pkg of 50 μg (11867423001)
pkg of 500 μg (11867431001)
제조업체/상표
Roche
동형
IgG1
에피토프 서열
YPYDVPDYA
저장 온도
2-8°C
관련 카테고리
일반 설명
Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
특이성
Anti-HA, High Affinity recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
면역원
Amino acids 98-106 from the human influenza virus hemagglutinin protein
애플리케이션
Use Anti-HA High Affinity for the detection of native influenza hemagglutinin protein and recombinant proteins that contain the HA epitope using:
- Dot blots
- ELISA
- Immunocytochemistry
- Immunoprecipitation
- Western blots
품질
Function tested in western blot.
제조 메모
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- ELISA: for detection 100 ng/ml; for coating 1 to 5 μg/ml
- Immunoprecipitation: 0.5 to 5 μg/ml
- Western blot: 50 to 200 ng/ml
재구성
Add 0.5 ml (11 867 423 001; 50 μg) or 2.5 ml (11 867 431 001; 500 μg) double-distilled water to a final concentration of 100 μg/ml or 200 μg/ml respectively.
Rehydrate for 10 min prior to use.
Rehydrate for 10 min prior to use.
기타 정보
For life science research only. Not for use in diagnostic procedures.
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신호어
Warning-Warning
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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